THP1 (1 × 105) or RAW264.7 (2 × 104) cells were plated onto the upper chamber for macrophage migration assay. The cells that migrated into the lower chamber media were collected and counted by the Bio-Rad (Hercules, CA, USA) TC10 automatic cell counter. Each sample was assayed in ...
The cells that migrated into the lower chamber media were collected and counted by the Bio-Rad (Hercules, CA, USA) TC10 automatic cell counter. Each sample was assayed in triplicate and the experiments were repeated twice. Cell invasion assay. PCa/macrophage cells were co-cultured in 0.4-mm...