Use of a tumour necrosis factor (TNF) antagonist for the preparation of a medicament for treatment of a TNF-mediated disease, wherein the TNF antagonist is to be administered as adjunctive therapy to methotrexate. Inventors: FELDMANN MARC (GB) MAINI NATH (GB) Application...
Brodacki B, Staszewski J, Toczyłowska B, Kozłowska E, Drela N, Chalimoniuk M, Stepien A (2008) Serum interleukin (IL-2, IL-10, IL-6, IL-4), TNF alpha, and INF gamma concentrations are elevated in patients with atypical and idiopathic parkinsonism. Neurosci Lett 441(2):158–...
ROS, reactive oxygen species; ER, endoplasmic reticulum; CRT, calreticulin; HSP70, 70-kDa heat shock protein; HSP90, 90-kDa heat shock protein; CD47, Cluster of Differentiation 47; HMGB1, high-mobility group box 1; ATP, adenosine triphosphate; TNF-α, tumour necrosis factor alpha; IFN-γ,...
TNF-alpha was purchased from PeproTech (Rehovot, Israel). Dexamethasone (Dex), 3-isobutyl-1-methylxanthine (IBMX), insulin, and lipopolysaccharide (LPS) were purchased from Sigma-Aldrich (St. Louis, MO, USA). All the primers for RT-qPCR were purchased from Bio Basic Inc., (Markham, ON...
Daidzein suppresses pro-inflammatory chemokine cxcl2 transcription in tnf-alpha-stimulated murine lung epithelial cells via depressing parp-1 activity. Acta Pharmacol. Sin. 2014, 35, 496–503. [CrossRef] [PubMed] 53. Tanaka, K.; Ohgo, Y.; Katayanagi, Y.; Yasui, K.; Hiramoto, S.; ...
Cytokine values lower than the limit of detection provided by BD-Biosciences (IL-6: 5 pg/mL; IL-10: 17.5 pg/mL; MCP-1: 52.7 pg/mL; IFN-γ: 2.5 pg/mL; TNFα: 7.3 pg/mL) were noted as not detectable (ND). 2.8. Statistical Analysis Crystal violet values were compared with a ...
Subsequently, samples were washed with BD Perm/Wash followed by intracellular staining in Perm/Wash reagent with anti-mouse GMCSF (clone MP1-22E9, BioLegend), IFN-γ (clone XMG1.2, Invitrogen), IL-2 (clone JES6-5H4, eBioscience), IL-17A (clone Tc11-1BH10.1, BioLegend), TNF-α (...
qPCR primer sets for tumor necrosis factor-α (TNF-α), interleukin (IL)-1β, IL-6, IL-8, intercellular adhesion molecule-1 (ICAM-1), glial fibrillary acidic protein (GFAP), the inducible form of nitric oxide synthase (iNOS), cluster of differentiation 68 (CD68), endothelial growth ...
Aqueous herbal extracts and isolated polyphenolic compounds (apigenin and quercetin) were incubated with THP1 macrophages to quantify interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α). The results showed that chamomile was the extract of lower anti-inflammatory potential and that both ...
Curcumin shows anti-inflammatory activity by binding directly to inflammatory molecules, such as tumour necrosis factor (TNF-α), cyclooxygenases COX-1 and COX-2, 5-lipoxygenase, 12-lipoxygenase, α1-human acid glycoprotein (α1-GA) and inducible nitric oxide synthase (iNOS) [5,6,7]. It ha...