Cells were imaged using 40 × (aperture 0.75) and 60 × (aperture 1.42) objectives with EVOS™ FL Auto 2 Imaging System (Invitrogen). Post-acquisition contrast adjustment was performed using the ImageJ software. A representative example for the quantification of bacteria co-localized within ...
DNA methylation is an important epigenetic mechanism involved in the anti-tumor immune response, and DNA methyltransferase inhibitors (DNMTi) have achieved impressive therapeutic outcomes in patients with certain cancer types. However, it is unclear how
Yang, Ivan Rodriguez Siordia, Jason Matthews, Michael Cohen,et al.alpha-Tubulin (T5168;Sigma-Aldrich), β-Actin (4967;CST), Lamin A/C (2032; CST), Histone H3 (4499;CST), FLAG (14793;CST), HA (3724;CST),GFP(A11122;Invitrogen), and mono/...
Fig. 7: TRABID targeting enhances the anti-tumor functions of PD-1 antibody. aSchematic presentation of the experimental workflow. C57BL/6 mice subcutaneously inoculated with B16F10 cells were injected with anti-PD-1 antibody and/or TRABID inhibitor as indicated.b,cTumor volume at indicated day...
Total RNA quantity and integrity were assessed, using Quant-IT RiboGreen RNA Assay Kit (Invitrogen, Carlsbad, CA, USA) and Agilent Tapestation 2200 RNA Screentape. Purification of mRNA, generation of double stranded cDNA and library construction were performed using TruSeq® Stranded mRNA HT (RS...
Sections were extensively washed in PBS and incubated for 3 h at RT in secondary antibodies all diluted 1:1000. The following secondary antibodies were used: donkey anti-goat Alexa Fluor 488, donkey anti-rabbit Alexa Fluor 568 or Alexa Fluor 647 (all Invitrogen/Life Technologies), donkey anti...
permeabilization and blocking in 0.2% Triton X-100 and 10% normal goat serum for 60 min. To label intracellular components, primary and secondary antibody incubation occurred sequentially for 1–2 h each. Prior to imaging, nuclei were stained with DAPI or NucBlue® reagent (Invitrogen™...
Anti-angiogenic therapies for cancer such as VEGF neutralizing antibody bevacizumab have limited durability. While mechanisms of resistance remain undefined, it is likely that acquired resistance to anti-angiogenic therapy will involve alterations of the tumor microenvironment. We confirmed increased tumor-as...
Protein arrays. ProtoArray Human Protein Microarrays v5.0 (Invitrogen, Carlsbad, CA, USA) were pur- chased and used according to the manufacturer's instructions. After blocking for 1 h at 4 °C and washing, arrays were incubated in quadriPERM dishes (Greiner Bio-One, Frickenhausen, ...
Data were generated by using Western blotting (pAMPKα1, ICAM1, Brn3 and GAP43), immunofluorescence (pAMPKα1, cd11b, TMEM119 and ICAM1), flow cytometry (CD11b, F4/80, CD68, CD206, IL-10 and LY6G), ELISA (TNF-α and IL-10) and transmission electron microscopy (TEM, for ...