Leukocyte contamination (< 2%) of PRP samples was estimated by flow cytometry, using the leukocyte-specific CD45 marker kit (Abcam, Cambridge, UK). Before use, WB and PRP samples were recalcified with a 200 mM CaCl2 stock solution to a final concentration of 5 mM. Isolated/washed ...
The IHC staining with the anti-elastin antibody was performed on the cross sections following Abcam IHC-paraffin protocol. (3) Hydration test: DPM 9003 device (NOVA, USA) attached to a 4-mm sensor probe was used to measure the impedance of EFT-412 skin tissues. Briefly, after two weeks...
Freshly harvested tumor cells were stained with antibodies against surface marker CD3 (ab16669, Abcam), CD4 (ab133616/ab183685, Abcam), CD8 (ab237709/ab217344, Abcam), CD11b (ab8878, Abcam), CD11c (ab219799, Abcam), CD15 (ab135377, Abcam), CD19 (ab245235, Abcam), CD49b (ab27...
Cells were washed twice and incubated for 60 min with goat anti-mouse IgG coupled to FITC (ab6785, Abcam, 1 μg/ml) and with DAPI (Hoechst 33258 pentahydrate (bis-benzimide), H3569, Invitrogen). Finally, cells were fixed a second time with paraformaldehyde 4% during 5 min....
Specific proteins such as FGA, fibrinogen beta chain (FGB), fibrinogen gamma chain (FGG) and vimentin (VIM) on the arrays were detected using antibodies: anti-FGA (CA-1023 cambio), anti-FGB (HPA001900 Sigma), anti-FGG (CA-1027-RA cambio) and anti-VIM (ab8978 abcam). Contra capture ...
Cells were washed twice and incubated for 60 min with goat anti-mouse IgG coupled to FITC (ab6785, Abcam, 1 μg/ml) and with DAPI (Hoechst 33258 pentahydrate (bis-benzimide), H3569, Invitrogen). Finally, cells were fixed a second time with paraformaldehyde 4% during 5 min....
After blocking with 5% (w/v) BSA (Baitg, Wuhan) in TBS (10 mM Tris-HCl (pH 8.0) and 150 mM NaCl) containing 0.1% Tween 20 at room temperature for 1 h, the membranes were then incubated with an appropriate specific primary antibody (Bcl-2 and Bax antibodies from Abcam, p53...
A cell counting kit-8 (CCK-8) was purchased from Dojindo Laboratories (Kumamoto, Japan). SP600125, SB203580, and PD98059 were obtained from Calbiochem (San Diego, CA, USA). Anti-TNF-α and anti-IL-6 were purchased from Abcam (Cambridge, MA, USA). Anti-β-actin and IL-1β were ...
(Dallas, TX, USA), MITF antibody (Ab-1) was obtained from NeoMarkers (Fremont, CA, USA), and antibody specific for TRP-1 was purchased from Abcam (Cambridge, UK). Antibodies specific for phospho-ERK1/2, total-ERK1/2, phospho-GSK3β, total-GSKβ, phospho-AKT, total-AKT, and β-...
For this, PE-labeled anti-human anti-FAP Ab (R&D Systems, Minneapo- lis, MN, USA) or rabbit anti human/mouse anti-FAP (Abcam plc, Cambridge, UK) and respective PE-labeled Fc-specific secondary Ab (Abcam plc, Cambridge, UK) were used. NB cells were detected using the Alexa Fluor647...