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Chronic low-grade inflammation contributes to the pathology and complications of type 2 diabetes (T2D). Interleukin-10 (IL10), an anti-inflammatory cytokine, is suggested to play a protective role in T2D. However, the impact of T2D on IL10 function has n
RNA was extracted from treated, infected cells using an RNeasy® Kit (Qiagen, Germantown, MD, USA) at 1, 2, and 4 h post treatment. Synthesis of cDNA for each RNA sample was accomplished using iScript™ cDNA Synthesis Kit (Bio-Rad, Hercules, CA, USA) using 1 μg of RNA ...
4.6. Apoptosis Detection by Flow Cytometry Annexin V-FITC and PI apoptosis kit (eBioscience™) was used to perform the apopto- sis detection. A total of 5 × 105 SH-SY5Y cells /mL were seeded into a six well plate and, after 24 h of incubation, they were treated with HTyr-OL, H...
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