The quantification of cytokines was performed by sandwich ELISA, using kits for detection of mouse TNF-α and IL-6 (R&D Systems, Minneapolis, MN, USA), according to the manufacturer's instructions. 2.5.6. Histopathology and Histomorphometry of the Small Intestine For histopathology and histo...
The individual mouse Quantikine® ELISA kits for IL-6 and TNF-α were obtained from R&D Systems (Minneapolis, MN, USA). Aurum total RNA mini kit, iScript™ Advanced cDNA Synthesis Kit,2-mercaptoethanol (14.2 M) 25 mL, ≥98% pure, SsoAdvanced™ Universal SYBR® Green Supermix, i...