Chronic low-grade inflammation contributes to the pathology and complications of type 2 diabetes (T2D). Interleukin-10 (IL10), an anti-inflammatory cytokine, is suggested to play a protective role in T2D. However, the impact of T2D on IL10 function has n
The supernatant (protein) was collected, and the protein concentration was determined using the Pierce™ BCA Protein Assay Kit. A series of concentration standards ranging from 0–2 mg/mL were prepared using bovine serum albumin (BSA). A total of 10 µL of each unknown protein sample and...
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2.6. Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR) After cell collection, total RNA was extracted using the RNeasy Plus Mini Kit (Qiagen, Milan, Italy). RNA concentration was detected with Nanodrop spectrophotometer ND-1000 (ThermoFisher) and 2 µg of RNA were reverse transcribed...
& Kit. is an herbaceous perennial plant, common from the steppe to the beech floor, growing in meadows, rare forests, rocky coasts, and ruderal places. A. setacea contains essential oils, flavonoids, alkaloids, glycosides, tannins, resins, organic acids, and vitamins C and K [2,3,4,5]...