After the colorimetric signal in the test and control lines stabilized, the detection patches were placed in a box, and pictures were taken using a camera (D5500; Nikon, Tokyo, Japan). The setup conditions, including the camera parameters, light source, and distance between the detection patch...
Sections were examined using an Axio Imager.Z1 ApoTome microscope (Carl Zeiss, Germany) equipped with a motorized stage and an AxioCam MRm camera (Zeiss). For confocal observation and analyses, an inverted laser scanning Axio observer microscope (LSM 710, Zeiss) with an EC Plan NeoFluor Â...
suspension was attached by the laser trap (run at low power not affecting the viability) to a 0.01% poly-L-lysine functionalized 9.7 mm microsphere (Duke Scientific Corp., Palo Alto, CA) that had been immobilized prior to adding sample by heating at 60uC for 1 h on a microscope cover ...
Single molecule data acquisition was carried out on the Nanoimager S running NimOS version 1.4 (Oxford NanoImaging). The images were acquired using a 100 × 1.4 NA Olympus objective, sCMOS camera (Hamamatsu orca flash 4.0 V3). 10,000 frames were recorded for the detection of Dylight550 ...
Micrographs were acquired every 30 sec for an observation period of 5 h using 63×/1.40 Plan-Apochromat oil-immersion objective and AxioCam HRm camera. The acquisition control and image processing were carried out using AxioVision 4.8.2 software (Carl Zeiss Microimaging, Germany). Disulfide ...
Classic endocytosis destinations include the recycling endosome returning to the plasma membrane or the late endosome (LE) merging with lysosomes for cargo degradation. However, the anti-angiogenic proteins angiostatin and isthmin, are endocytosed and tr
(CCD) camera mounted on an upright microscope, and analyzed by a Fiji imaging macro software. The live cell percentage, according to the detected fluorescence intensity, was normalized against the untreated cells. The IC50values were fitted and evaluated by a four-parameter logistic equation ...
The image acquisition was performed with Leica DM2000 Fluorescence microscope (Leica Microsystems, Milan, Italy) equipped with Leica DFC450 C digital camera (Leica Microsystems). Positive cells for the markers were counted and reported as a percentage of positive cells in the bar chart. Total cell...
Lipids were stained red. The stained aortas were spread on a black charpie for photographing using a digital camera under identical light condition with same photographing parameters as much as possible. Pictures were loaded to Image Pro Plus (Version 6.0, Media Cybernetics, Washington DC, USA)....
“Methods”). Underlain blue-yellow curve shows raw data; black curve gives 15-point smoothed average.bColor visualization of R/G/B data acquired with a line-scan camera (Methods).cDeep-sea isotopic stack LR04 for the past 1.5 Ma23.dAtmospheric CO2records derived from a composite ice core...