Detection of Anti-Red Blood Cell Antibodies by Treatment of the Cells with Neuraminidasedoi:10.1111/j.1600-0609.1977.tb02078.xA. KatzA. KlajmanA. YaretzkyZ. P. SteinerAhuva KnyszynskiBlackwell Publishing Ltd
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On days 14, 18, 21, and 26, 50 μL of peripheral blood was collected by using tail-clip method and then treated with ACK lysis buffer (Cambrex) for 5 minutes to deplete the red blood cell. Cells were stained with αCD3, αCD8, and P1A tetramer antibodies. For IFN-γ intracellular ...
Some have hypothesized that perhaps anti-endothelial cell antibodies, which are present in the circulation in several autoimmune conditions, may be responsible for the injury. Several studies have correlated the presence of anti-endothelial cell antibodies with the development and severity of IPF (Magro...
Pure Red Cell Aplasia Induced by Antibodies against Human Recombinant Erythropoietin Human recombinant erythropoietin (epoetin) is a very effective and safe biopharmaceutical. However, in 1998 the incidence of pure red cell aplasia (PRCA) i... A Krüger,Eckardt, Kai-Uwe - 《Transfusion Medicine & ...
The following primary antibodies were purchased from Cell Signaling Technology and used at a dilution of 1:1000: AMPK-α (#2532), Phospho-AMPKα (Thr172) (#2531), Acetyl-CoA carboxylase (#3662), and Phospho-Acetyl-CoA carboxylase (Ser79) (#3661). After incubation with the secondary ...
Blood-brain Barrier Transporters Cell Structure and Signaling Molecules Cytokine and Growth Factor Receptors on VSMC Diabetes Diabetic Peripheral Neuropathy Glucose Homeostasis Immune Regulatory Molecules on Endothelial Cells Inflammatory Mediators Integrins and Associated Molecules ...
While all cancer cell lines were growth inhibited by MF, only the breast cancer MCF-7 cells expressed cognate PR. Conclusions Antiprogestin MF inhibits the growth of different cancer cell lines with a cytostatic effect at lower concentrations in association with a decline in the activity of the ...
Pure red-cell aplasia and anti-erythropoietin antibodies in patients treated with recombinant erythropoietin. N Engl J Med. 2002;346:469–75.N. Casadevall. Pure red cell aplasia and anti- erythropoietin antibodies in patients treated with epoetin. Nephrol Dial Transplant, 18 Suppl 8:viii37-viii...