Anti-PE micro beads 操作方法
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Compensation Beads FC Related Pages & Pathways Pages Quality Control Related FAQs What type of PE do you use in your conjugates? We use R-PE in our conjugates. Other Formats View All Phycoerythrin Reagents Request Custom Conjugation DescriptionCloneApplications Purified anti-phycoerythrin (...
Figure 1.Compensation using the AbC™ Anti-Mouse Bead Kit. (A) Phycoerythrin (PE)-conjugated mouse anti-human CD56 antibody (MHCD56044) labeling the AbC™ capture beads for a positive signal and negative beads providing a negative signal. (B) FITC...
(Miltenyi Biotec). The CD8+ T cells were activated with CD3/CD28 magnetic beads for 3 days, and upregulation of CD137 was monitored by flow cytometry. 50,000 T cells were added to CHO-PD-L1 cells at a ratio of 1:10 (CHO-PD-L1 to T cells), previously plated on CellView Cover...
Figure 1.Compensation using the AbC™ anti-Rat/Hamster Bead Kit. (A) FITC-conjugated rat anti-mouse CD4 (MCD0401) labels the AbC™ RH capture beads for a positive signal and the negative beads provide a negative signal. (B) Phycoerythrin ...
Purity:The antibody was purified from antisera by a combination of papain digestion and immunoaffinity chromatography using antigens coupled to agarose beads. Fc fragments and whole IgG molecules have been removed. Buffer:0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6 ...
For immunoisolation method, protein A/G agarose beads pre-bound with anti-Rab5 (EE marker)/anti-Rab7 (LE marker) antibodies were used to pull down EE/LE in the supernatant, respectively [49]. For sucrose step-gradient centrifugation method, the supernatant is then subjected to sucrose step ...
Purified 6xHis-SUMO-Int (1000 nM) and/or untagged PexA (250 nM) were added to Novex Dynabeads His-Tag Isolation & Pulldown beads with Binding/Wash buffer (50 mM Sodium-phosphate pH 8, 300 mM NaCl, 0,01% Tween-20) and incubated rocking at room temperature for 10 min. Unb...