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It has been reported that VEGFR-3 also takes part in tumor angiogenesis, and blocking VEGFR-3 signaling with a highly specific VEGFR-3-TKI or monoclonal antibody has shown ability to reduce vascular density.36,40,41 In the current work, we employed two VEGFR-3-TKIs to verify our hypothesis...
This epitope was selected from a study identifying binding epitopes of the pathological tau-specific monoclonal antibody DC8E8, which exhibited no off-target binding48. Considering the difficulty of extracting defined soluble oligomers from the brain and other tissues, TauRD could serve as an ...
Use of chemotherapy plus a monoclonal antibody against HER2 for metastatic breast cancer that overexpresses HER2. N Engl J Med 2001; 344: 783–792. Article CAS PubMed Google Scholar Geyer CE, Forster J, Lindquist D, Chan S, Romieu CG, Pienkowski T et al. Lapatinib plus capecitabine for ...
Rabbit monoclonal anti Ki67 was purchased from Thermo Sientific (MA, USA). Immunoblotting procedures Details of immunoblotting have been described previously28. Monoclonal antibody against GAPDH was purchased from Santa Cruz Biotechnology (Santa Cruz, USA). Rabbit polyclonal anti EVA1A antibody was ...
(d) anti- RAINBOW monoclonal antibody does not cross-react with unstained cellular proteins. 1: E. coli; 2: A. thaliana; 3: S. cerevisiae; 4: C. elegans; 5: D. melanogaster; 6: Chicken follicle; 7: CHO, Chinese hamster ovary; 8: Rat1, rat fibroblasts; 9: N2a, mouse ...
The rabbit anti- CTGF monoclonal antibody (E-5) was obtained from Santa Cruz. The anti-GAPDH (13E5) monoclonal antibody was obtained from Cell Signaling Technology. The GAPDH levels served as internal controls. Animals, Induction of Pulmonary Fibrosis and Treatment. All animal procedures were...
The secondary antibodies used were Alexa Fluor 488 goat anti-mouse IgG and Alexa Fluor 555 goat anti-rat IgG. Negative controls were generated by omitting the primary antibody. In addition, rat IgG2A (R&D Systems) was used as an isotype control for the rat anti-rat RAGE monoclonal antibody....
12,24,28 Demonstration of antibody responses against FHVH33-CD8BBZ was somewhat unexpected; however, this finding is similar to prior reports of recipient antibody responses against fully human monoclonal antibodies.78 Most anti-myeloma activity occurred in the first 2–4 weeks after FHVH-T in...
After incubation with peroxidase-conjugated donkey anti-mouse IgG antibody (1:1000; Abcam, UK) for 2 h at room temperature, sections were developed using diaminobenzidine (DAB; Vector Laboratories, USA) and counterstained with haematoxylin (Solarbio, China). As for NF-κB immunoreactivity detection...