However, only epitopes displayed on the pVIII protein were still recognized by BSW17 whereas pIII‐expressed epitopes were rapidly inactivated. Surprisingly, when used for oral immunization of mice all phage clones induced anti‐IgE antibodies. In contrast, oral immunization with the purified, pVIII...
A phage display library (PhDTM-12) expressing 12-mer peptides fused to a minor coat protein (pIII) of a non-lytic bacteriophage (M13) was purchased from New England Biolabs (Cambridge, MA). A purified form of HS isolated from bovine kidney was purchased from Sigma. Soluble 3-OS HS modi...
FIG. 7 depicts the phagemid construct for surface display of Fab-pIII fusions on phage. The phagemid encodes a humanized version of the Fab fragment for antibody A4.6.1 fused to a portion of the M13 gene III coat protein. The fusion protein consists of the Fab joined at the carboxyl ...
the helper phage M13KO7 was added to the culture when bacteria were grown up to the log-phase,in which scFv antibody was displayed in N-terminus of the bacteriophage coat protein pIII.The phage display antibody was then applied to four rounds of "panning-enrichment-amplification" to screen ...
mitogen-activated protein kinase; pIII, wild-type gene III protein of filamentous phage; PBS, phosphate buffered saline; PEG, polyethylene glycol; scFv, single-chain antibody fragment; TBS, tris buffered saline; TCS, tissue culture supernatant. ...
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