For NK-cell depletion, 300 µg of anti-NK1.1 antibody (clone PK136; BioXCell) was intraperitoneally administered in 100 µL of HBSS every 2-3 days starting 1 day before the first vehicle/LNP intratumoral injection. For neutrophil depletion, mice received 75 µg anti-Ly6G ...
anti-mouse Ly-6G, PE (clone 1A8) BD Biosciences Cat # 551461; RRID:AB_394208 anti-mouse Siglec-F, APC (clone 1RNM44N) eBioscience Cat # 50-1702-82; RRID:AB_2574187 anti-mouse IL-4, APC (clone 11B11) BD Biosciences Cat # 557739; RRID:AB_397256 anti-mouse IL-13, PE (clone...
148Nd-anti-ICOS (Clone C398.4A) Fluidigm Cat#3148019B; RRID: AB_2756435 149Sm-anti-CD69 (Clone H1.273) Biolegend Cat#104502; RRID: AB_313105 150Nd-anti-Ly6G (Clone 1A8) Biolegend Cat#127601; RRID: AB_1089179 150Nd-anti-CD73 (Clone TY/11.8) Biolegend Cat#127202; RRID: AB_10...
(clone RM4-5), anti-mouse CD8a (clone 53-6.7), anti-mouse CD45 (clone 30-F11), anti-mouse Gr-1 (clone RB6-8C5), anti-mouse CD11b (clone M1/70), anti-mouse Ly6G (clone 1A8), anti-mouse Ly6C (clone AL-21), anti-mouse F4/80 (clone BM8) and anti-mouse CD11c (clone N...
Chimeric agonist anti-mouse CD40 antibody (mIgG1, clone 3–23) sequences were obtained from professor Martin Glennie (University of Southampton, England). Codon optimization for the production in HEK (Homo sapiens) and CHO (Cricetulus griseus), as well as sub-cloning into pCEP4_A164 expression...
MP, Palmer E, Haqq CM, Carter C, Fontenot J, Villinger F, McNeil LK, DeMuth PC.1:40),CD3(AF700,clone: 17A2,BioLegendcat#100216,1:100),CD19(AF700,clone: 6D5,BioLegendcat#115528,1:100),Ly6C(BV650,clone: HK1.4,BioLegendcat#128049,1:125),Ly6G(PerCP-Cy5.5,clone: 1A8,B...
BD Horizon™ Alternative Name Ly6c, Lymphocyte antigen 6C2; Lymphocyte antigen 6G, Ly6g, Gr-1 Concentration 0.2 mg/ml Isotype Rat IgG2b,κ Reactivity Mouse (QC Testing) Application Flow cytometry (Routinely Tested) Immunogen Not Reported Storage Buffer Aqueous buffered solution containing BSA, ...
Chimeric agonist anti-mouse CD40 antibody (mIgG1, clone 3–23) sequences were obtained from professor Martin Glennie (University of Southampton, England). Codon optimization for the production in HEK (Homo sapiens) and CHO (Cricetulus griseus), as well as sub-cloning into pCEP4_A164 expression...
4/3/2018Staining Mouse Splenocytes With Anti-B220 PE/Cy7Adam GuessThis antibody was used as a B cell marker in mouse splenocytes. It was chosen because it is a commonly used clone for B220, PE/Cy7 is bright and works well with other fluorophores in the staining ...Read Review ...
Ly-6G (Clone 1A8) BioLegend Cat#127602; RRID: AB_1089180 CD69 (Clone H1.2F3) Invitrogen Cat#14-0691-82; RRID: AB_467325 CD4 (Clone RM4-5) BioLegend Cat#116018; RRID: AB_2650936 F4/80 (Clone BM8) BioLegend Cat#123102; RRID: AB_893506 Eomes (Clone Dan11mag) Invitrogen Cat#14...