小鼠 抗-Ly-6G 磁珠超纯化开发用于从小鼠骨髓,肺和血液的单细胞悬液中正选或去除小鼠中性粒细胞。 小鼠抗-Ly-6G 超纯化微磁珠用于从淋巴组织中直接,阳选或去除小鼠中性粒细胞。 Ly-6G在嗜中性粒细胞上高表达,在嗜酸性粒细胞亚群中表达较低,在单核细胞发育阶段短暂表达。 该试剂盒含有抗Ly-6G克隆REA526,其与...
优惠:请询价 订货号:2ZQ0257 品牌型号:美天旎 130-120-337 货期:询货期 最小订货量:1件 +- 美天旎 联系我们 400-920-3909 微信扫一扫,手机采购 产品介绍Product Description The Anti-Ly-6G MicroBeads UltraPure, mouse were developed for positive selection or depletion of mouse neutrophils from single-...
(9001–12-1, BIOFROXX, Germany) at 37 °C for 1 h. A 70 µm cell mesh filter was used to filter the tissue-lysate mixture. The cells were incubated with anti-Ly-6G MicroBeads (Miltenyi Biotec, Bergisch Gladbach, Germany) for 30 min at 4 °C. Using magnetic separation columns (...
pooled, and CD45.2+ OT-I memory T cells were isolated using an EasySep Mouse CD8+ T cell Isolation kit, followed by column isolation using biotinylated anti-mouse CD45.2 (BioLegend, 109804), LS columns (Miltenyi Biotec, 130-042-401) and anti-biotin MicroBeads (Miltenyi Biotec, 130-090-...
(Miltenyi Biotec). Separation of Ly6G+and Ly6G−cells relied on magnetic bead isolation using anti-Ly6G MicroBeads UltraPure (Miltenyi Biotec). In short, up to 2 × 108Ly6G+cells in the single cell suspension of primary tumors were magnetically labelled with MicroBeads, after which the ...
(3) TF modulation to enhance CAR8 T cell function may depend on epigenetic and transcriptomic contexts determined by prior antigen experience. Prior work has shown dose-dependent effects in the antitumor responses of adoptively transferred T cells2, and CAR T cells have been shown to elicit ...
Splenocytes were harvested from Mettl3fl/fl-Ncr1Cre/+ mice or Mettl3fl/fl mice, and incubated with anti-NKp46 mAb (Biolegend, San Diego, CA, USA) at 4 °C, followed by labeling of anti-PE MicroBeads (Miltenyi Biotec, Bergisch Gladbach, Germany). Then NK cells were sorted with a...
Cell suspensions were incubated with MACS CDllb MicroBeads (Miltenyi Biotec, Auburn, CA, USA) and separated by positive selection using MS columns. Immunofluorescence For whole lung tissue staining of IKFM and control mice, lungs were first perfused with PBS, then inflated and fixed in formalin ...
Lymphocytes were isolated from the spleen and peripheral lymph nodes, and then CD4+T cells were enriched by using CD4 (L3T4) Microbeads (130-049-201, Miltenyi Biotec). Then CD4+CD25hiYFP+Treg cells and CD4+CD25−YFP−Tconv cells were sorted on a BD FACS Aria II cell sorter (BD Bio...
2.2. Single Antigen Beads Assay with Single-HLA Antigen-Coated Microbeads To monitor IgG reactivity to HLA-A, HLA-B, HLA-C, HLA-E, HLA-F, and HLA-G, the culture supernatants as well as human sera, were analyzed with SAB assay using dual-laser flow cytometry on a Luminex platform (...