a = control, normal leech, treated with seawater only, b = leech treated with 0.25 % of formalin solution, c = leech treated with methanol extract of neem leaves. 2.4. Physio-chemical parameters Physio-chemical parameters including temperature, salinity, pH and dissolved oxygen in the solutions...
Medicinal leech therapy (Hirudotherapy) : A brief overview Hirudotherapy is a treatment using medicinal leeches. Hirudo medicinali, have been used to treat patients for centuries. In the past, leeches have proved t... AP Singh - 《Complement Ther Clin Pract》 被引量: 72发表: 2010年 ...
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CONSTITUTION: Salivary gland of Haementeria ghilianii of leech is surgically removed and homogenized to give tissue homogenate, which is extracted with a solution of ammonium sulfate, acetone, etc. The extract is concentrated or dehydrated, chromatographed on DEAE cellulose, an anion exchange resin...
(Nissl staining). After being defatted for 15 min in 100% xylene and 10 min in 100% ethanol then rehydrated, sections were stained in 0.1% cresyl violet solution and mounted in DePeX mounting medium (VWR). Images were acquired with a Keyence BZ-9000 microscope. Hippocampal volume was ...
tissues and species using immunoblotting (42kDa). The antibody cross-reacts with β-Actin expressed in cells of human, bovine, sheep, pig, rabbit, cat, dog, mouse, rat, guinea pig, chicken, carp, and Hirudo medicinalis (leech) tissues, but not inDictyostelium discoideumamoebae orDrosophila...
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