a, Schematic of L-PLA using fucosylated HLA-DRB1 (fuco-HLA-DRB1) as an example. We stained for (1) HLA-DRB1 using anti-HLA-DRB1 antibody followed by oligonucleotide-conjugated PLA secondary antibody (2° Ab) (+) and (2) fucosylated glycan using biotinylated (‘B’) AAL lectin followed ...
Hendrickx DAE, van Eden CG, Schuurman KG, Hamann J, Huitinga I (2017) Staining of HLA-DR, Iba1 and CD68 in human microglia reveals partially overlapping expression depending on cellular morphology and pathology. J Neuroimmunol 309:12–22. https://doi.org/10.1016/j.jneuroim.2017.04.007 Art...
In the presence of human serum, the anti-HLA-DR antibody (solid circles) mediated significant cell lysis, while incubation with HuLuc63 (solid squares) or isotype control antibody (open diamonds) exerted no effect. In the absence of human serum, the anti-HLA-DR antibody also lacked cell ...
Reactive microglia in patients with senile dementia of the Alzheimer type are positive for the histocompatibility glycoprotein HLA-DR. Neurosci Lett. 1987;79(1–2):195–200. Article CAS PubMed Google Scholar El Khoury J, Toft M, Hickman SE, Means TK, Terada K, Geula C, Luster AD. Ccr2...
ANTI-HLA-DR-FITC/CD3-PE ANTI-HUMAN KAPPA LIGHT CHAINモノクローナル抗ヒトΚL鎖(結合型および遊離型)抗体 マウス宿主抗体 ANTI-MOUSE IGG2A, BIOTIN ANTI-MOUSE CD5, BIOTIN ANTI-HUMAN CD8 BIOTIN ANTI-RAF-1 ANTI-CADHERIN-5抗VE-カドヘリン抗体 ウサギ宿主抗体 ...
The following assessments were included in this study: questionnaires for allergy history, family history, complete medical history and exposure (environmental and ingestion) history, chemistry, hematology and urinalysis panels, hepatitis screening, anti-PEG antibody determination, and HLA typing. No ...
Expanded TCR clones had higher expression of activation (i.e., GZMB, IFNG, HLA-DR, CCL3) and immune checkpoint markers (i.e., HAVCR2, LAG3, CTLA4) (Figure S14C). Expanded TCRs in responders but not the non-responder showed upregulation of CD137 (TNSFR9, 4-1BB), a co-...
Circulating immunosuppressive phenotypes were compared: mean proportions of CD4+CD25+FoxP3+ cells (Treg) (0.95 ± 0.5 % vs 0.88 ± 0.3 %, p = 0.89), and CD11b+CD33+HLA-DR−CD83− cells (myeloid-derived suppressor cells (MDSC)) (0.6 ± 0.1 % vs 0.9 ± 0.3 %, p = 0.34) did ...
To vary the ratio of antibody coating on beads equimolar solutions of anti-CD3 and anti-CD28 were mixed at 1:0, 1:5, 1:10, 1:20, 1:40, 1:80, 2 1:160, and 0:1 ratios. Control beads were coated with biotinylated IgG1 isotype. Coating was performed on a rotator stand at room ...
The elutions were controlled by werethcino-nlatyroerllcehdrombyatotghrianp-hlayyaecrcocrdhirnogmtoattohgermapethhyodafrcocmordVienngnattoandthceollmeaegtuheosd[7].frTohmeseVaeunthnoarts and colleiadgeunetisfi[e7d].fTrahcetisoensauotfhdoirfsfeirdeennt tmifioeldecfurlaacrtiwoenisghotf danifd...