J ChIP assays were performed with an anti-H3K9ac antibody at the Il9 locus in Th9 cells. The intensities of these modifications relative to input DNA were determined via quantitative RT‒PCR analysis. n = 3 ( E , F , H , I , J ); ( C , D , G ) for each group, biologically...
The membranes were then incubated overnight at 4°C with primary antibodies against AcH3K9 (1:6000; Abcam, Cambridge, MA), histone H3 (1:20000; Abcam), FOXO3 (1:2500; Abcam), Bax (1:5000; Abcam), Bcl-2 (1:2000; Abcam) and glyceraldehyde-3-phosphate dehydrogenase (1:5000; Santa ...
To determine if glutarate also inhibits KDM enzymes, we cultured CD8+T cells with DEG for 7 days and determined the methylation status of a number of histone 3 (H3) lysine residues (Fig.2f). DEG treatment of CD8+T cells increased dimethylation and trimethylation of H3K9 and trimethylation of...
(17)YTHDC1 negatively regulates UBE3A to influence RAD51 ubiquitination and inhibit apoptosis in colorectal cancer cellsSci RepMarch 14, 2025Mingwei Gao, Yueguang Wu, Li Zhang, Yan Zhou, Huijuan Liu, Weimin Zhang, Shubin Wang, Yongping Cui(Proteintec...
厂家:Abcam公司 规格:100ug 保存温度:-20℃ 和 2～8℃ 货期:现货2～5天,期货2-3周 咨询:(重庆) (贵阳) Anti-Histone H3 (tri methyl K36) antibody - ChIP Grade (ab9050)产品信息: Product nameAnti-Histone H3 (tri methyl K36) antibody - ChIP GradeSee all Histone H3 primary antibodies ......
it can either recruit the histone demethylase KDM3B to erase the repressive histone mark H3K9me2, or dictate nuclear RNA decay to restrict the chromatin activity and downstream transcription23,24,25. These discoveries raise questions about whether the m6A machinery adapts the binding interface for co...
Following primary antibodies were used: General H3 (Sigma, H0164), H3K4me1 (Abcam, 8895), H3K4me2 (Abcam, 32356), H3K9Ac (Abcam, 69830), H3K9acS10P (Sigma, H9161), H3K18ac (Cell signaling, 9675), H3K27ac (Abcam, 45173), H3K36me3 (Sigma, SAB4800028), H3K56ac (Sigma, SAB...
Acetylation is generally linked to gene activation. Acetylation on Lys-10 (H3K9ac) impairs methylation at Arg-9 (H3R8me2s). Acetylation on Lys-19 (H3K18ac) and Lys-24 (H3K24ac) favors methylation at Arg-18 (H3R17me). Citrullination at Arg-9 (H3R8ci) and/or Arg-18 (H3R17ci) by...
it can either recruit the histone demethylase KDM3B to erase the repressive histone mark H3K9me2, or dictate nuclear RNA decay to restrict the chromatin activity and downstream transcription23,24,25. These discoveries raise questions about whether the m6A machinery adapts the binding interface for co...
The following primary antibodies were added sequentially on the slides: rat anti-mouse F4/80 (clone CI:A3-1, Bio-Rad, Hercules, CA, USA), rat anti-mouse Ly6C (clone ER-MP20, Abcam, Cambridge, UK), rabbit anti-mouse Ly6G (clone EPR22909-135, Abcam), rat anti-mouse CD8a (4SM15, ...