Besides flow cytometric immunophenotyping, single cell suspensions of 4T1 primary tumors were also subjected to cellular separation for downstream protein analysis. More specifically, CD45−and CD45+cells were separated on a MACSQuant Tyto Cell Sorter (Miltenyi Biotec). Separation of Ly6G+and Ly6G...
was able to exhibit significant inhibition at high concentrations in both cell lines. It is thought that the fact that theC. pichlerisubsp.pichlerimethanol extract is higher than the others in terms of cytotoxic activity may be due to its higher total phenol and total flavonoid contents and it...
To date, the biological properties of cedar oil from Lebanese cedar (Cedrus libani) found in Lebanon and south-central Turkey have not been widely described. However, published data indicate that it exhibits antibacterial and antifungal activities [23,31,32]. Therefore, this paper is a report ...
The 1O2 generation capacity of GRDs was determined by ABDA probe. 4T1 cells were incubated with 24 µg/mL of RB or GRDs for 24 h and irradiated at a wavelength of 532 nm for 5 min (30 mW/cm2), and cell mortalities of 63.5% and 36.4% were obtained for GRDs, and 36.4% for...
Cell depletion was performed by means of weekly i.v. injection of 30 μg anti-mouse CD8α (clone 2.43, BioXCell, Lebanon, NH, USA) diluted in PBS. For adoptive T cell transfer, naïve CD8 T cells were isolated from spleen and lymph nodes of Cor93 TCR-transgenic mice by negative mag...