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Kun Yang, Ivan Rodriguez Siordia, Jason Matthews, Michael Cohen,et al.alpha-Tubulin (T5168;Sigma-Aldrich), β-Actin (4967;CST), Lamin A/C (2032; CST), Histone H3 (4499;CST), FLAG (14793;CST), HA (3724;CST),GFP(A11122;Invitrogen), and...
MCF-7 cells from ATCC were routinely cultured in phenol red free-RPMI medium (Invitrogen Corporation, Scotland, UK), 10% FCS (Invitrogen Corporation), 1% L-Glutamine (Invitrogen Corporation). MCF-7 cells were plated in 96-well tissue culture plates at a density of 5×104 cells/well in ser...
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with a fluorescent secondary antibody (Molecular Probes A21207 or A11055) for 1 h at 37 °C in a humified chamber. Antibody solutions were made in PBS (Sigma). Following labelling procedures, samples/cells were mounted on glass slides in Clear Mount mounting solution (Invitrogen). ...
Cells were stained with either fixable eFluor780 Live/Dead stain (eBioscience) or aqua live/dead viability stain (Invitrogen) at 4°C in PBS. For cell surface staining antibodies were incubated with cells in the dark for 30 min at 4°C in PBS/1% BSA + 10% FCS (GIBCO). Intracellular ...
After low MOI infection, cells infected with adenovirus-GFP exhibited prominent green fluorescence and STING-cherry aggregates (Figure 6E). STING-cherry aggregates were also detected in uninfected cells identified by the absence of green fluorescence. These cells were often physically separated from ...
125 ng of pcDNA-PB1, pcDNA-PB2, pcDNA-PA, pcDNA-NP, pPolI-Luc-RT and pEGFP were co-transfected to HEK293T cells to reconstitute the RNP complexes with Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA). Then, growth medium with selected compounds were added after 6 h of transfection....