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深圳子科生物科技有限公司并代理各大进口原装品牌,代理*各地约余300种各*,具体有:R&D、IBL、BD、Roche、Sigma、Santa、Tsz、CST、Amresco、Sciencell、Qiagen、Omega、Immonoway、Wako、Abcam、Merk、ATCC、Axygen、Invitrogen、Gibio、Hyclone、Cloud-clone corp、MP、Novus 、 lifespan Exqion、GE 、clontech 、...
Cells were lysed in pH 7.5 buffer containing 50 mM Tris, 150 mM NaCl, 2 mM EDTA, 50 mM NaF, 1% Nonidet-P40 and 2 mM Na4P2O7 plus protease inhibitors (Roche). Cell lysates were loaded on NuPAGE® Novex® 4–12% Bis-Tris Protein Gels, 1.0 mm, 10 well (NP032...
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4a), while the lateral roots of wag1 wag2 double mutants shifted to a more vertical GSA orientation, similar to WT (Extended Data Fig. 4b), indicating that auxin might control lateral root GSA through an RCN1-dependent pathway. We also observed that PIN3:GFP polarity was shifted towards ...
Primers were designed using National Center for Biotechnology Information (NCBI) GeneBank sequences (for primer sequences, see supplementary Table 1). The GAPDH gene and β-actin gene were utilized as an endogenous control. The LightCycler 480 system (Roche Diagnostics) was used to perform qPCR....
本产品用于GFP-Flag融合蛋白的免疫沉淀效果参考图2。图2. BeyoMag™ Anti-Flag Magnetic Beads (Anti-Flag磁珠)用于GFP-Flag融合蛋白的免疫沉淀效果图。293T (人胚肾细胞)转染GFP-Flag质粒36小时后,经Western及IP细胞裂解液(P0013)裂解。样品1为Input,即全细胞裂解液(total);样品2为Mouse IgG Magnetic Beads ...
GFP-labeled HUVEC cells were grown in EBM-2 basal medium and EGM-2 singleQuots supplements. Lids of 10 cm cell culture dish were seeded with 20 μl growth medium containing 400 cells and then inverting culture dish lid. For hydration of the drops, 10 ml PBS were added to the bo...
Roche/罗氏 AXYGEN Peninsula/美国 BioGenex Cedarlane Greiner Applikon ZEISS/蔡司 Leica/徕卡 产品简介 供货周期 现货规格 盒 货号 130-096-435应用领域 医疗卫生,环保,化工,生物产业,制药/生物制药 主要用途 科研单位实验用 德国美天旎生物技术公司(Miltenyi Biotec GmbH)是一个以细胞分选技术为主、拥有多样化产品...
CCR4 levels were evaluated by flow cytometry (FACSCalibur, BD biosciences) using cell surface labeling of EGFP with monoclo- nal mouse anti-GFP (Roche Applied Science; 1:100 dilution) as primary antibody and R-phycoerythrin conjugated AffiniPure F (ab')2 fragment goat anti-mouse (Immunotech; ...