Anti-FLAG M2 Magnetic Beads(抗FLAG M2 磁珠),1ml分享给好友: 详细描述 目录号:M8823-1ML 品牌:Sigma-Aldrich 规格:1ML 目录价:￥4132.44 市场价格:￥4132.44 会员价格:￥4132.44 到货时间:多数为现货, 2-3个工作日 数量: 购买发起团购 结束时间: ...
M8823-5ML Anti-FLAG® M2 Magnetic Beads5ml 产品说明: 一般描述 抗-FLAG M2磁珠是可与抗-FLAG M2(小鼠单克隆)抗体结合的4%琼脂糖微珠。 该M2抗体可识别FLAG序列的N-末端、Met N-末端和C-末端。 这可实现对含有FLAG肽序列的融合蛋白的检测和捕获。
Sigma货号M8823,纯度/规格 支Anti-FLAG M2 Magnetic Beads 详细介绍 该磁性属性可实现:- 极为快速的分离- 显著被加速的操作,例如重复的洗涤-以板式进行多个样本的处理该可导致:- 更为快速的实验- 更好的可重复性- 目标蛋白更为准确的定量 我公司产品有多种纯度,规格。需要了解或订购更多产品、规格、技术信息请...
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Anti-FLAG M2 Magnetic Beads are 4% agarose beads bound with the exclusive Sigma Anti-FLAG M2 (mouse monoclonal) antibody.
25 ng of N-terminal DYKDDDDK-tagged GFP protein was spiked into A549 lysate and captured using Pierce Anti-DYKDDDDK Magnetic Agarose or Sigma Anti-FLAG™ M2 Magnetic Beads using the KingFisher Flex Purification System. Tagged proteins were eluted with IgG Elution Buffer and analyzed by western bl...
For immunoprecipitation, cell lysates containing an equal amount of proteins were incubated with anti-Flag agarose beads (Sigma-Aldrich), GFP-Trap agarose beads (Chromotek), or antibody-conjugated Protein A Magnetic beads (Merck) at 4 °C for 2 h. The beads were washed with lysis buffer...
The mixed lysate was incubated with anti-FLAG magnetic beads (MBL, M185-11) at 25 °C for 40 min. After removing RNA-binding proteins from the FLAG beads with phosphoserine phosphatase, the mixture was incubated with fresh pre-washed SpyCatcher beads at 25 °C for 1 h. Stringent...
To determine the amount of assembled diabody, conditioned media from trans- fected HEK-293 cells were purified with Ni-NTA magnetic beads and the eluates were analyzed by western blotting using anti- FLAG mAb. As shown in Figure 2b, the amount of assembled αCEA ×αCD3 diabody in ...
The supernatant was obtained by lysing the cells with the NP-40 lysis buffer (KeyGene Biotech) containing phosphatase and protease inhibitors. Then, the supernatant was mixed with anti-Flag, anti-c-Myc, or anti-HA antibody at 4 °C overnight. The protein A/G magnetic beads (MedChemExpre...