Make sure the bottle of ANTI-FLAG M2 affinity gel is a uniform suspension of gel beads. Remove an appropriate aliquot for use. 4. Immediately transfer the suspension to the column. 5. Allow the gel bed to drain and rinse the pipette used for the resin aliquot with TBS. The 50% ...
Anti-Flag Affinity Beads, 由高品质的小鼠Flag单克隆抗体与sepharose 4B beads共价偶联制成。本产品具有高载量(至少为1mg Flag标签融合蛋白/ml gel),高特异性性,杂蛋白的非特异性结合较少,可用于细菌和哺乳动物细胞裂解物以及体外表达系统中 Flag 标记蛋白的免疫沉淀 (IP) 实验,也可用于免疫共沉淀 (Co-IP) 和...
EZviewTM Red Protein A and ANTI-FLAG® M2 Affinity Gels: Immunoprecipitation with Enhanced Visibility Affinity BeadsThe strength and specificity of interactions between biomolecules have been exploited for years in biochemical research. Affinity-based protein purification techniques have been used ...
Anti-FLAG® M2 Magnetic Beadsaffinity isolated antibody Synonym(s): Monoclonal ANTI-FLAG® M2 antibody produced in mouse, FLAG® magnetic affinity resin, FLAG® resin for high throughput, Flag® Affinity resin, Anti-ddddk, Anti-dykddddk...
初步的western blot结果显示有目的蛋白产生，而且beads也能纯化目的蛋白，但我始终无法用3×flag peptide...
Affinity Pull-Down of Proteins Using Anti-FLAG M2 Agarose Beads. E Gerace,D Moazed - 《Methods in Enzymology》 被引量: 0发表: 2015年 Formation of a Carboxy-Terminal Domain Phosphatase (Fcp1)/TFIIF/RNA Polymerase II (pol II) Complex in Schizosaccharomyces pombe Involves Direct Interaction......
Anti-Flag Affinity Gel (Anti-Flag亲和凝胶)由高质量的鼠源Flag抗体与琼脂糖共价偶联而成,可与常规蛋白表达系统(如细菌、酵母或哺乳动物细胞)中含有Flag标签的蛋白结合,从而用于带有Flag标签的融合蛋白或蛋白复合物的纯化或免疫沉淀(Immunoprecipitation, IP)。
Anti-c-Myc Affinity Beads可以检测原核、真核表达的c-Myc标签融合蛋白。c-Myc 多肽是由人类染色体8q24.上的c-Myc基因编码,对应于人类P62的410-419 (EQKLISEEDL) 氨基酸。Anti=c-Myc Affinity Beads可以识别C端,N端或内部c-Myc标签蛋白融合蛋白,可应用在Western-blot 杂交技术、免疫沉淀和流式细胞计量术中,用...
Anti-DYKDDDDKAffinity Beads是以抗flag(DYKDDDDK)抗体为亲和配体,一步纯化原核、酵母或哺乳动物细胞表达的flag标签融合蛋白。Anti-DYKDDDDK Affinity Beads以4%琼脂糖凝胶为基质,杂蛋白非特异性结合少,可用于flag标签融合蛋白的纯化和免疫沉淀(IP)。 表1.Anti-DYKDDDDKAffinity Beads产品性能...
在本研究中,研究者使用了Biolinkedin®Anti-Flag affinity gels进行免疫沉淀(immunoprecipitation,IP),检测p62S的泛素化修饰(Fig.1f)。 同时,使用了Biolinkedin®Anti-Flag Magnetic beads进行了免疫共沉淀(Co-immunoprecipitation,Co-IP)实验,检测TRIM72与p62S的相互作用(Fig.S3a) ...