Hsc70-WT-Flag or Hsc70-3KA-Flag for 8 h, the co-localization between RAB7A and PD-L1 (I), the co-localization between LAMP1 and PD-L1 (J), the co-localization between RAB11 and PD-L1 (K) were done using immunofluorescence and confocal microscopy. Scale bar, 5 μm...
(Bimake, added fresh), and incubated with anti-Flag magnetic beads (after washing the beads with PBS twice) for 6 h on a rotating wheel at 4 °C. The beads were washed with TAP buffer three times and treated with CIP (#M0290, Biolabs) at 37 °C for 30 min. The kinase ...