When the anti-ssDNA antibody (Ab) test is used for the differential diagnosis of new patients suspected of an inflammatory rheumatic disease the clinical sensitivity for systemic lupus erythematosus (SLE) is close to 100%, and the specificity about 85%. Since anti-dsDNA (double stranded DNA) ...
Clinical value of anti-ssDNA (denatured DNA) autoantibody test: beauty is in the eyes of the beholder The level of anti-DNA antibodies varies in different SLE patients’ plasma, with high levels of anti-ssDNA and/or anti-dsDNA antibodies being associated ... M Teodorescu - 《Clinical & App...
Overall, 42.4% (n = 48), 27.4% (n = 31), and 78.8% (n = 89) of patients with selected clinical conditions were positive for DNA-ELISA, DNA-CLIFT, and ANA-IIFA anti-dsDNA Ab screening, respectively (Table 1). Table 1. General results of DNA-ELISA, DNA-CLIFT and ANA-IIF for se...
dsDNA antibodies and ANuA in diagnosing SLE were evaluated taking ELISA as standard.Results:The positive coincident rate of immunoblotting for anti-ds-DNA antibodies and ANuA was 73.33% and 66.28%,respectively.The positive coincident rate of crithidia luciliae immunofluorescence test for anti-ds-DNA ...
Ungulaviridae (Captovirus AFV1), Bicaudaviridae (SMV2, SMV3, SMV4, STSV1 and STSV2), Fuselloviridae (SseV isolate 1), an unclassified dsDNA virus (SYV1) and six MAGs (JAKMAA010000023.1, JAKMAB010000038.1, JAEMPR010000070.1, JAEMPR010000099.1, JAEMPR010000147.1, and JAEMPR010000199.1). ...
Baseline laboratory profiles, encompassing parameters such as anti-dsDNA antibody (Anti-dsDNA ab), C3, C4, white blood cell count (WBC), neutrophils, basophils, monocytes, eosinophils, hemoglobin, erythrocyte count, hematocrit, mean corpuscular volume, mean corpuscular hemoglobin, mean corpuscular ...
DNA fragments, library purity and concentration were assessed using a Qubit 3.0 Fluorometer and dsDNA HS Assay kit (Invitrogen, Waltham, MA, United States). Fragment length was determined on a 4200 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). cfDNA-seq was performed using a targeted...
In the past, anti-dsDNA antibodies were detected by enzyme-linked immunosorbent assay (ELISA) [14], Crithidia luciliae immunofluorescence test (CLIFT) [15], and radioimmunoassay [16]. However, new methods have been developed in the last decades such as fluorescent enzyme immunoassay (FEIA) [17...
Both clinicians (82%; n = 246) and laboratory professionals (81%; n = 352) considered the combination of an ANA IIF test result with a corresponding specific anti-ENA or anti-dsDNA test result as the most clinically relevant information. The quantification of the nuclear ANA IIF fl...
Analysis of the potency of the equine anti-SARS-CoV-2 F(ab′)2immunoglobulin The potency of anti-SARS-CoV-2 hyperimmune serum was assessed using three different methodologies: competitive ELISA (GenScript) described as a surrogate virus neutralization test based on antibody-mediated blockage of AC...