Sequences of both the heavy and the light chain for each antibody variant were confirmed by Sanger sequencing. Plasmids for each antibody variant were transfected into Expi293 cells (Invitrogen) supernatant at a 1:3 heavy:light containing secreted ratio. After antibodies incubating at was collected...
Lewis1,3 & Marzena Pazgier1,2 Antibody-dependent cell-mediated cytotoxicity (ADCC) by non-neutralizing antibodies (nnAbs) specific to the HIV envelope (Env) glycoproteins present at the surface of virus sensitized or infected cells plays a role in the effective adaptive immune response to...
(Invitrogen, Carlsbad, Calif., USA), using the murine monoclonal IgG1 antibody 6E10 (Covance, Emeryville, Calif., USA) that recognizes all Aβ42.conformers. Binding of the antibody was visualized using a goat anti-mouse IgG antibody that was conjugated to horseradish peroxidase (HRP, Sigma,...
The sections were then stained with polyclonal rabbit anti-rat IgG (1:200, Dako), or EnVision rabbit (Dako) with NOVAred substrate (Vector). The tissue sections were counterstained with hematoxylin. The percentage of CD8 + or Ki67+ cells were quantified with Qupath software [51], using...
Electrophoretic mobility shift assay was carried out as previously described.13 3 Western blot Western blots were performed as previously described.36 The membranes were incubated overnight at 4 °C with rabbit polyclonal anti-Bcl3 antibody (1:500, Santa Cruz Biotechnology), caspase-3 (1:1000, ...
Mouse GFAP (1:200; Sigma-Aldrich, St. Louis, MO, USA) was used to stain astrocytes, with Alexa Fluor 568-labeled goat anti-mouse IgG (H + L, 1:300; Invitrogen) as the secondary antibody. A mounting medium was used (Vectashield; Vector Laboratories). Images of the hippocampus were ...
molecules Article Steppogenin Isolated from Cudrania tricuspidata Shows Antineuroinflammatory Effects via NF-κB and MAPK Pathways in LPS-Stimulated BV2 and Primary Rat Microglial Cells Dong-Cheol Kim 1, Tran Hong Quang 2, Hyuncheol Oh 1,3 and Youn-Chul Kim 1,* 1 Institute of Pharmaceutical ...
cancers Article EBV Positive Diffuse Large B Cell Lymphoma and Chronic Lymphocytic Leukemia Patients Exhibit Increased Anti-dUTPase Antibodies Marshall Williams 1,2 and Maria Eugenia Ariza 1,2,* 1 Department of Cancer Biology and Genetics, The Ohio State University College of Medicine, Columbus, OH ...
then washed with a suitable detergent-containing buffer such as phosphate buffered saline (PBS)/Tween, followed by the addition of the components of a suitable detection system. For example, horseradish peroxidase conjugated goat antihuman kappa chain polyclonal antibody is added, followed by washing,...
IgA antibody conjugated to horseradish peroxidase HRP (AbD serotech AA140P), in a dilution of 1/10,000 in 2% skimmed milk, was used. While detecting the plant-produced VHH-IgG antibodies, 100 μl of polyclonal anti-porcine IgG produced in rabbit (Sigma A5670) conjugated to HRP diluted 1...