The proteins were diluted in a 2x Laemmli buffer at a 1:1 ratio and denatured by heating the samples at 100 °C for 5–10 min. Equal amounts of protein (15–30 µg) were loaded into the wells of a Tris-glycine gel (4–15%), followed by protein separation at 100 V for 85 ...
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As a result, a large number of new quinoxaline 1,4-dioxides are being synthesized and their biological properties tested each year. One important feature for their biological activities is the presence of both N-oxide functional groups in the pyrazine ring of the basic quinoxaline structure. N-...