Abcam recommends using 3-5% milk as the blocking agent ChIP Use at an assay dependent concentration. IHC-P 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. Target Anti-Histone H3 (citrulline R2 + R8 + R17) antibody - ...
The αB7-H3-αCD3 ENG-T cells demonstrated significant anti-tumor activity against MM cell lines expressing B7-H3. SupT-1 cells (B7-H3neg) served as controls and exhibited minimal cytotoxicity from αB7-H3-αCD3 ENG T cells. In contrast, these engineered T cells showed dose-dependent killi...
13 To probe for specific proteins, primary antibodies for SLPI (1:4000, ab17157, Abcam, RRID:AB_443693), AAT (1:800, A0012, DAKO, RRID: AB_2335672), NE (1:100, MAB91671, R&D systems) and citrullinated histone H3 (1:2000, ab5103, abcam, RRID:AB_304752) were used in 4% normal...
and separated by SDS-PAGE and probed with appropriate antibodies: anti-FGA (CA-1023 Cambio, Cambridge, United Kingdom), anticitrullinated fibrinogen antibody (MQ13.102 Modiquest, Moleneind, The Netherlands), antihistone H3 XP (4499 Abcam, Cambridge, MA) and anti-cit-Histone H3 (ab5103 Abcam)...
Lineage transitions are a central feature of prostate development, tumourigenesis and treatment resistance. While epigenetic changes are well known to drive prostate lineage transitions, it remains unclear how upstream metabolic signalling contributes to the regulation of prostate epithelial identity. To fill...
13 To probe for specific proteins, primary antibodies for SLPI (1:4000, ab17157, Abcam, RRID:AB_443693), AAT (1:800, A0012, DAKO, RRID: AB_2335672), NE (1:100, MAB91671, R&D systems) and citrullinated histone H3 (1:2000, ab5103, abcam, RRID:AB_304752) were used in 4% normal...
International Journal of Molecular Sciences Article Post-Translational Protein Deimination Signatures in Serum and Serum-Extracellular Vesicles of Bos taurus Reveal Immune, Anti-Pathogenic, Anti-Viral, Metabolic and Cancer-Related Pathways for Deimination Michael F. Criscitiello 1,2, Igor Kraev 3 and...
International Journal of Molecular Sciences Article Anti-Inflammatory Effects of Chloranthalactone B in LPS-Stimulated RAW264.7 Cells Xueqin Li 1,†, Jun Shen 2,†, Yunyao Jiang 3,4,†, Ting Shen 5,8, Long You 5,8, Xiaobo Sun 6, Xudong Xu 6, Weicheng Hu 5,8,*, Haifeng Wu 6...
The GSH level (GSH/GSSG ratio detection assay kit, Abcam, Cambridge, UK) was detected at the Ex/Em of 490 nm/520 nm. Nutrients 2018, 10, 1368 4 of 17 2.8. Measurement of Mitochondrial Membrane Potential (MMP, ∆Ψm) The mitochondrial membrane potential (MMP, ∆Ψm) was determined...
(Cat No.: 610299) was purchased from BD Pharmingen, San Jose, CA, USA; peNOS (Cat No.: 66127) from Abcam, Cambridge, MA, USA; VEGF-B (Cat No.: MAB751) and VEGF165b (Cat No.: MAB3045), used in Western blotting as well as to block VEGF165b in vitro and in vivo, was ...