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CD8+ OT-I T cell isolation was performed using a mouse CD8a+ T cell isolation kit (Miltenyi) according to manufacturer’s instructions. Isolated CD8a+ OT-I T cells were labeled with 5 μM CFSE (Sigma). Successful CFSE staining was confirmed by flow cytometry. In vitro OT-I T cell ...
CD8+ T cells were depleted in a subset of mice by intraperitoneal injection of CD8a depletion antibody (BioXcell, 2.43 clone), 3 times per week at 150 μg per animal. Drugs were given for the duration of the study, and comparable control treated with vehicle or rat IgG2a isotype ...
Thus GDF-15, beside its known role in cancer-related anorexia and cachexia, emerges as a regulator of T cell extravasation into the tumor microenvironment, which provides an even stronger rationale for therapeutic anti-GDF-15 antibody development....
Upon an average volume of 200 mm3, mice were randomized in different treatment groups: RT + PBS + IgG (LFT-2, BioXcell), RT + L19-IL2 + IgG, RT + PBS + aPD-L1 (10F.9G2, BioXcell), and RT + L19-IL2 + aPD-L1. Tumors were locally irradiated with 5 Gy (CT26) or 10 Gy ...
Using single-cell RNA sequencing, we found that APG-2575 polarized M2-like immunosuppressive macrophages toward the M1-like immunostimulatory phenotype with increased CCL5 and CXCL10 secretion, restoring T-cell function and promoting a favorable immunotherapy response. Mechanistically, we demonstrated that...
CD8a (Clone 53-6.7) BioLegend Cat#100737; RRID: AB_10897101 CD45 (Clone 30-F11) BioLegend Cat#103138; RRID: AB_2563061 PerCP-anti-TER119 BioLegend Cat#116225; RRID: AB_893637 inVivoMAb anti-mouse PD-L1 (B7-H1) Bio X Cell Cat#BE0101; RRID: AB_10949073 inVivoMAb anti-mouse ...
Cells were stained with the following antibodies (BioLegend, San Diego, USA) for 30 min at 4 °C: anti-mouse CD3 (clone 145-2C11), anti-mouse CD4 (clone RM4-5), anti-mouse CD8a (clone 53-6.7), anti-mouse CD45 (clone 30-F11), anti-mouse Gr-1 (clone RB6-8C5), anti-...