viruses or substances released from injured or dying cells that alarm the immune system to danger. CD14 has the unusual property of existing both on the surface of white blood cells and floating free in the blood and lung fluids. This dual existence allows CD14 to amplify the body's inflammat...
anti-human CD4-BrilliantViolet711 clone A161A1, anti-human CD19-BrilliantViolet711 clone HIB19, anti-human CD56-APC clone 5.1H11, anti-human CD66b-PECy7 clone G10P5 (all from BioLegend), anti-human CD8-PE clone UCHT-4 and anti-human CD14-FITC clone MEM-15 (both from Immunotools, ...
Therapy also markedly altered the monocyte compartment, significantly upregulating type 1 interferon and interferon gamma pathways in CD14+ monocytes, and decreased the percent of CD16+ monocytes, suggestive of potential trafficking to the tumor. Collectively, our data demonstrates that N-803 plus ...
To study the safety, tolerability and anti-viral activity of the combination of two broad and potent anti-HIV-1 antibodies, 3BNC117 and 10-107416,17in the presence or absence of ART, we conducted a phase 1b, open-label, randomized clinical trial of two treatment groups of chronically infec...
d, Differential expression of the monocyte markers CD206 and CD14 after different treatments. B cell (e) and dendritic cell subsets (f) in patients did not change substantially after treatment. In b-f, n=17 in Pre-DDP, n=19 in Post-DDP; n=15 in Pre-BEV, n=16 in Post- BEV; n=...
PBMCs were isolated using Ficoll-Paque (GE Healthcare) from fresh buffy coats of healthy anonymized donors at the Blood Centre at Uppsala University Hospital. PBMCs were cultured in RPMI-1640 supplemented with 10% FBS, 1 mM sodium pyruvate and 1% PeSt. CD14+cells in the PBMC pool were ...
Glimepiride reduces CD14 expression and cytokine secretion from macrophages J. Neuroinflamm., 11 (2014), p. 115 View in ScopusGoogle Scholar [28] J. Kim, J.H. Park, K. Shah, S.J. Mitchell, K. Cho, H.S. Hoe The anti-diabetic drug gliquidone modulates lipopolysaccharide-mediated microgli...
CD14+ cells in the PBMC pool were isolated using specific beads (Miltenyi Biotec) and differentiated over 5 d into immature DCs (imDCs) in the presence of 50 ng ml−1 granulocyte-macrophage colony-stimulating factor (Gentaur) and 25 ng ml−1 IL-4 (Gentaur). Fresh medium ...
Bukovsky observed that niche of FGSCs formed during early embryonic development consist of nonspecific ovarian monocyte-derived cells(MDCs), T cells, and vascular endothelial cells, whereas nests of adult ovarian germinal stem cells consist of primary CD14 + MDCs, activated HLA-DR + M...
The CD14+ monocytes were isolated by positive selection using CD14 microbeads (130–118-906, Miltenyi Biotec, Auburn, CA, USA) according to the manufacturer’s instructions. The CD14+ selected cells were cultured in RPMI 1640 (Gibco) containing 10% FBS (Gibco), 2 mM L-glutamine (Gibco), ...