PRMT5-activated c-Myc promote bladder cancer proliferation and invasion through up-regulating NF-κB pathway. Tissue Cell. 2022;76:101788. Article CAS PubMed Google Scholar McKean M et al. A phase 1 dose escalation study of protein arginine methyltransferase 5 (PRMT5) inhibitor PRT543 in ...
The following primary antibodies were used: anti-SDMe-arginine (Cell Signaling Technology, 13222), anti-MYB (Cell Signaling Technology, 12319), anti-MYC (Abcam, ab32072), anti-AXL (Abcam, ab215205), anti-AKT (Cell Signaling Technology, 4691 S), anti-phospho-AKT (Cell Signaling Technology,...
Then, we examined the levels of c-Myc protein in three MM cell lines after anlotinib treatment, and the results showed that anlotinib dramatically down-regulated c-Myc in a dose- and time-dependent manner without effect on its mRNA levels (Figs.4EandS3). Thus, these results indicated that ...
The membranes were blocked with 3 % BSA in TBS at room temperature for 1 hour. Primary antibodies—p-γH2AX (ab11174), c-Myc (ab32072), BCL-2 (ab32124), BCL-XL (ab2568), CDK1 (ab131450), p-CDK1 (ab47594), Cyclin B1 (ab32053), mTOR (ab32028) from Abcam; CDC25C ...
Wargon V, Riggio M, Giulianelli S, Sequeira GR, Rojas P, May M, Polo ML et al (2015) Progestin and antiprogestin responsiveness in breast cancer is driven by the PRA/PRB ratio via AIB1 or SMRT recruitment to the CCND1 and MYC promoters. Int J Cancer 136(11):2680–2692. doi:10.10...
Fig. 3 CDK7 regulates the p38α/MYC pathway in NSCLC. a Changes of RNAPII C-terminal domain (CTD) phosphorylation and apoptosis markers in A549 and H460 cells after vehicle or THZ1 treatment at 48 h. b KEGG pathway analysis of differential expression genes for THZ1(200 nM, 24 h)...
Ab3 is the anti-idiotypic antibody to Ab2. Because of the chain of structural complementarity, Ab1 and Ab3 can have similar specificity for the original antigen. Antibodies have been used in the past in tumor immunotherapy in two ways: Ab1 antibodies as tumor-specific antigens on B ...
and limited studies have shown that the expression of NDRG1 is inhibited by Myc, and some chemical inducers such as NO, nickel (Ni2+) and cobalt (Co2+), as well as chemical iron chelators, can induce its expression58,59,60,61. Suppressed expression of NDRG1 significantly reduces cell ...
In CD166-HA-transfected Bel-7402 cells, YAP was immunoprecipitated with an anti-FLAG antibody, and Western blotting analysis of TEAD was done by an anti-Myc antibody. L, YAP intracellular localization in Bel-7402 cells with or without CD166-HA overexpressed. Scale bar, 15 μm. M, no ...
Another LOXL inhibitor to show early promise in cancer treatment was the anti- LOXL2 monoclonal mouse antibody AB0023, which was effective in reducing intratumoral collagen density in vivo in a murine model of pancreatic cancer [157]. Following from this, a humanized version of this antibody, ...