1 vial containing 1 ml each of Mouse anti-APC Nanobeads Product Details Verified Reactivity Mouse Formulation Aqueous solution containing BSA and 0.05% sodium azide. Preparation Conjugated particles: protein-coated magnetic beads. Storage & Handling ...
Resuspend the beads by vortexing, maximum speed, 5 touches. Add the appropriate volume of Human anti-APC Nanobeads to the cell suspension, mix well and incubate on ice for 15 minutes. The volume of MojoSort™ Human anti-APC Nanobeads should be adjusted depending on starting percentage of cell...
美天旎 Miltenyi 130-092-344 CD203-APC, human 4,176.90 美天旎 Miltenyi 130-092-345 CD203c-Biotin, human 3,258.45 美天旎 Miltenyi 130-092-355 CD69 MicroBead Kit II, human 7,706.79 美天旎 Miltenyi 130-092-357 Anti-Biotin MACSiBeads, cell cult.-grade 8,145.54 美天旎 Miltenyi 130-092...
BD™ CompBeads can be used as surrogates to assess fluorescence spillover (Compensation). When fluorochrome conjugated antibodies are bound to CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral ...
上海玉博生物科技有限公司 主要致力于“Anti-APCDD1(adenomatosio polyposis coli down-regulated 1) 腺瘤**肉调节蛋白抗体 yb-1565R”的生产销售。多年的“Anti-APCDD1(adenomatosio polyposis coli down-regulated 1) 腺瘤**肉调节蛋白抗体 yb-1565R”生产与销售的
Purity:The antibody was purified from antisera by a combination of pepsin digestion and immunoaffinity chromatography using antigens coupled to agarose beads. Fc fragments and whole IgG molecules have been removed. Buffer:0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6 ...
可以用不同浓度的咪唑进行梯度洗脱,在样品及平衡缓冲液中添加0.5%吐温或Triton X-100,或5~50%的甘油,可以避免因为疏水相互作用导致非特异吸附,或使用钴离子螯合磁珠(BeaverbeadsTM IDA-Cobalt)。此外,要获得纯度较高的蛋白,需要对裂解、结合缓冲液、洗涤缓冲液、洗脱缓冲液的组分、pH、咪唑浓度等进行优化。若还是...
Compensation controls were prepared using 0.5 µg ml−1 of each antibody with UltraComp eBeads (Thermo Fisher Scientific). After staining, cells were washed, fixed (2% formaldehyde), washed and subjected to flow cytometric analysis. Dendritic and CD4+ T cell subpopulations were subjected ...
Flow cytometer with blue and red lasers capable of measuring phycoerythrin (PE) and allophycocyanin (APC) channels Aluminum foil or 96-well Plate Aluminum Sealers Distilled water 1.5 ml polypropylene microcentrifuge tubes or similar Microcentrifuge ...
T cells were spinoculated with retroviral supernatant on plates coated with Retronectin (Clontech, T100B). On day +3, beads were removed and %GFP was measured by flow cytometry to assess the efficiency of gene transfer. Cells were either used fresh or frozen and thawed as needed for the ex...