Ils sont hétérogènes, se fixant sur la bande A et I du sarcomère et reconnaissent la myosine, l’actine, l’α-actinine, la titine, la filamine, la vinculine, la tropomyosine et le canal ryanodine du réticulum sarcoplasmique (Yamamoto et al., 1987, Ohta et al., 1990, My...
Briefly, 3 μm sections of paraffin-embedded kidneys were submitted to antigen heat retrieval and incubated with anti-FMNL1 antibody (Abcam) at 1/200 dilution 1 h at room temperature followed by an anti-rabbit antibody for 30 min (room temperature) and then liquid diaminobenzidine substrate–...
Secondary antibodies of horseradish peroxidase (HRP)-conjugated anti-rabbit IgG (L3012) and HRP-conjugated anti-mouse IgG (L3032) were obtained from Signalway Antibody (Maryland, USA). 2.2. Animal models and treatments Male C57BL/6 J mice (3 weeks old) were purchased from Shanghai SLAC ...
β-actin PCR product is indicated as an internal control. Sham-operated (SO); Ischemia/reperfusion (I/R); Pioglitazone (Pio); 5-hydroxydecanoate plus pioglitazone (5-HD + Pio). Compared with I/R, Pio induced a decreased expression of P38MAPKmRNA and JNKmRNA (p < 0.05). There...
www.nature.com/scientificreports OPEN Anti-high mobility group box-1 (HMGB1) antibody inhibits hemorrhage-induced brain injury received: 28 November 2016 accepted: 09 March 2017 Published: 10 April 2017 and improved neurological deficits in rats Dengli Wang1, Keyue Liu1, Hidenori Wake1, ...
This antibody is specific to a membrane-proximal CD19 epitope and harbors faster on/off rates compared to FMC63. We tested h1218-CART19 in vitro and in vivo using FMC63-CART19-resistant models. We conducted a first-in-human multi-center phase I clinical trial to test AT101 (clinical-...
Stripped blots were washed 6 times in TBST, blocked, and reprobed with an alternative antibody. Statistical analysis Data is presented as mean ± SD unless reported otherwise. The progression of tumor development in different dietary groups was compared using the Chi-square test, whereas the ...
αSMA: α-Smooth muscle actin ADAM: A disintegrin and metalloproteinase ACT: Adoptive cell transfer Ang: Angiopoietin CAR: Chimeric antigen receptor CTL: Cytotoxic T lymphocyte CTLA-4: Cytotoxic T-lymphocyte-associated protein 4 DLK1: Delta-like homologue 1 ...
Antibodies p-p65, p65, ZO-1, and occludin were obtained from Shanghai Abcam Co., Ltd., β-actin was obtained from Wuhan Sevier Biotechnology Co., Ltd, the secondary antibody goat anti-rabbit IgG-HRP was obtained from Jiangsu Kangwei Century Biotechnology Co., Ltd, DSS (molecular weight 36...
To determine the direct effects of ritonavir on activated caspases-1, -3, and -8, PBMCs were stimulated with anti-CD3 monoclonal antibody (mAb) and subsequently primed to apoptosis using CD95-agonistic mAbs (clone CH-11; 0.5 mg/L; Kamyia, San Francisco, CA). Cell lysates were prepared ...