Trans-neuronal viral tracing is becoming one of the most important methods for mapping neuronal circuit connections,but an anterograde trans-synaptic tracer compatible with functional assays is still lacking.Adeno-associated virus(AAV)is replication-defective,and its production of offspring needs the help...
Anterograde transsynaptic tracing in the murine somatosensory system using Pseudorabies virus (PrV): A "live-cell"-tracing tool for analysis of identified ... (2007). "Anterograde transsynaptic tracing in the murine somatosensory system using Pseudorabies virus (PrV): A "live-cell"- tracing tool...
A Mutant Vesicular Stomatitis Virus with Reduced Cytotoxicity and Enhanced Anterograde Trans-synaptic Efficiency Vesicular stomatitis virusAttenuated cytotoxicityEnhanced anterograde trans-synaptic tracingUnderstanding the connecting structure of brain network is the basis to ... K Lin,X Zhong,M Ying,... -...
Figure 1. Advantages of Anterograde Transsynaptic Mapping of Functional Circuits (A) A brain region “X” is known to mediate a behavior/function of interest. Neurons in X project to multiple target nuclei (“Y”). To map the relevant downstream circuit, conventional method relies on activation...
Within the primary visual pathway, we found that TRAS-M51R significantly improved long-term viability of transsynaptic labeling (compared to TRAS) while maintaining anterograde spread activity. By using Cre-expressing VSV(M51R)ΔG, TRAS-M51R could selectively label excitatory ( vglut2a positive) ...
leading to efficient synapse-specific and anterograde-only transneuronal spreading, which can be controlled to achieve either monosynaptic or polysynaptic tracing. Moreover, inducible transient replication of YFV-17D mutant is sufficient to induce permanent transneuronal genetic modifications without causing...
Transneuronal tracingAnterogradeRetrogradeRecombinantsFluorescent reportersDual infectionThe use of viruses as transneuronal tracers has become an increasingly powerful technique for defining the synaptic organization of neural networks. Although a number of recombinant alpha herpesviruses are known to spread ...
Mapping neural circuits is critical for understanding the structure and function of the nervous system.Engineered viruses are a valuable tool for tracing neural circuits.However,current tracers do not fully meet the needs for this approach because of various drawbacks,such as toxicity and ...
Trans-neuronal viral tracing is becoming one of the most important methods for mapping neuronal circuit connections,but an anterograde trans-synaptic tracer compatible with functional assays is still lacking.Adeno-associated virus(AAV)is replication-defective,and its production of offspring needs the help...
Here we describe the use of vesicular stomatitis virus (VSV) for tracing neuronal connections in vivo. We made VSV vectors that used glycoprotein (G) genes from several other viruses. The G protein from lymphocytic choriomeningitis virus endowed VSV with the ability to spread transsynaptically,...