The Annexin V-FITC Apoptosis Detection Kit Plus includes annexin V-FITC, SYTOX green dye, and binding buffer. The SYTOX green dye is impermeant to live cells and apoptotic cells, but stains necrotic cells with intense green fluorescence by binding to cellular nucleic acids. After staining a ...
Annexin V iFluor® conjugates are useful for live-cell imaging, immunofluorescence, and flow cytometry. They can be combined with other dyes, such as nucleic acid stains, to accurately assess mixed populations of apoptotic, necrotic, and dead cells. AAT Bioquest offers a wide variety of ...
AnnexinVlabeledwithafluorophoreorbiotincanidentifyapoptoticcellsbybindingtoPSexposedontheouterleaflet.10TheAlexaFluor®488annexinV/DeadCellApoptosisKitwithAlexa®Fluor488annexinVandPIforflowcytometryprovidesarapidandconvenientassayforapoptosis.ThekitcontainsrecombinantannexinVconjugatedtooneofourbestandbrightest...
在流式细胞分析期间,使用含 Annexin V 偶联物(包括 Alexa Fluor 488、FITC、碘化丙啶、PE、APC 和 SYTOX Green)的死细胞凋亡试剂盒可轻松区分活细胞、死细胞和凋亡细胞。这些偶联物通过不同的染料区分活细胞、死细胞或凋亡细胞,这对于使用多参数研究确认细胞活力和细胞凋亡非常重要。 使用用于进行流式细胞分析的含 ...
采用用于细胞凋亡检测的 Annexin V 独立偶联物,可快速而可靠地检测早期细胞凋亡。使用流式细胞分析,Annexin V 偶联物在凋亡细胞与非凋亡细胞之间提供达 100 倍的荧光信号强度差异。 膜联蛋白 V 对磷脂酰丝氨酸 (PS) 具有高亲和力,PS 可暴露于正在凋亡的细胞的外叶上。由于具有这种亲和力,荧光标记的膜联蛋白 V ...
In addition, the assay can be directly performed on; Live cells, without the need of fixation. The Annexin V-FITC Apoptosis Detection Kit Plus includes annexin V-FITC, SYTOX green dye, and binding buffer. The SYTOX green dye is impermeant to; Live cells and apoptotic cells, but stains ...
Annexin V-FITC apoptosis detection kit plus FITC-labelled recombinant human annexin V shows bright green fluorescence. SYTOX green dye is impermeant to live cells and apoptotic cells, but stains necrotic cells with intense green fluorescence (Ex(max): 488nm; Em(max): 530nm). Apoptotic cells sho...
Cells must be analyzed immediately. Dead cells are stained by propidium iodide, which has excitation and emission maxima at 535 nm and 617 nm, respectively. Early stage apoptotic cells stained by Annexin V FITC can be detected using a filter designed to detect fluorescein (excitation/emission = ...
the cell membrane loses integrity, thereby allowing annexin V to also access PS in the interior of the cell. A viability dye can be used to resolve these late-stage apoptotic and necrotic cells (annexin V, viability dye-positive) from the early-stage apoptotic cells (annexin V positive, viab...
to; Live cells and apoptotic cells, but stains necrotic cells with intense green fluorescence by binding to cellular nucleic acids. After staining a cell population with annexin V-FITC and SYTOX Green dye in the provided binding buffer, apoptotic cells show green fluorescence, dead cells show a ...