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It has been suggested that the physical presence of the HDR template at the time and location of the nuclease event facilitates a higher frequency of template-guided repair. If the physical presence of the template in closeness, proximity to the DSB at the time of its creation improves HDR, ...
Be careful not to guess at where sections are glued — always match numbers when gluing. For example, if you guess, you may think that #18 and #26 get glued right next to the sections beside them, but in fact they join up with #18 and #26 on the outer ears. ...
The cDNA template for activin bA, including T7 Translational Psychiatry and SP6 promotors for sense and antisense riboprobe in vitro transcription, was generated by PCR amplification from the vector using a T7 primer (50-GAATTGTAATACGAC TCACTATAGGGCGAATTG-30) and an SP6 primer (50-CC A...
The cDNA template for activin βA, including T7 and SP6 promotors for sense and antisense riboprobe in vitro transcription, was generated by PCR amplification from the vector using a T7 primer (5′-GAATTGTAATACGACTCACTATAGGGCGAATTG-3′) and an SP6 primer (5′-CCAAGCTATTTAGGTGACACTATAGA...
gRNA was transcribed from a template oligo as previously described46. The sequence of the selected Crispr target site was 5′-GAGTGGAGCTGGAAAAACAGAGG-3′ (bold lettering indicates the PAM motif). The efficiency of Crispr/Cas9-induced mutagenesis was monitored at 24 hpf using a T7 ...
some techniques of trajectory estimation are presented in [4]: (a) two differential techniques by means of background subtraction; (b) entropy thresholding proceeding by using a global and local threshold; and (c) color matching with a computed template integrating a selected spectrum of colors....
The validated anti-EBOV GP IgG ELISA method [56] was used as a template for the anti-MARV and anti-SUDV GP IgG ELISA method development. All buffers, incubation periods, temperatures, and handling procedures were the same as those used for the anti-EBOV GP IgG ELISA. ...
Optional Instruction: Add Quilt Batting to the Lining –Do this when stitching the lining pieces together.(1)Cut 5 pieces of batting using the outside pattern piece. I used my cut fabric as a template.(2)Place tape over the open edges of your zig-zag foot. This ...