We started coming to Animal Care, Ipswich, MA when we moved here over 10 years ago. We have gone to Beverly Animal Hospital, Beverly Animal Natural Health Center and Danvers Animal Hospital. They all seemed the same. Then we met Dr Carlson, he was straight forward and always got to the...
At Hamilton Wenham Veterinary Hospital, we offer remarkable animal health care services to clients in Hamilton, MA. Get in touch with us.
NEBNext Ultra DNA library prep kit for Illumina libraries (New England Biolabs #E7645L, Ipswich, MA) was used for library construction, selecting for 150–200 bp (H3K4me3, H3K27ac, CTCF) or 200–400 bp (H3K27me3, H3K4me1) insert fragment sizes using Ampure beads (Beckman Coulter...
Just off Faunce Corner Road in Dartmouth is an animal sanctuary for livestock that has become home to over 50 animals is just five short years. Whether they arrived because their owners could no longer care for them or they were removed from an abusive situation, Deborah Devlin and Jill Tagi...
For tau analysis, extracts of the hippocampus were incubated with alkaline phosphatase mix (500 mM Tris–HCl pH 9.0, 500 mM MgCl2, 0.1 M DTT (Invitrogen), 10,000U/ml calf intestinal alkaline phosphatase (New England Biolabs, Ipswich, MA)) at 37 °C overnight for dephosphorylation. Subsequen...
Linearized DNA templates were enzymatically transcribed into RNA with T7 RNA polymerase (New England Biolabs, Ipswich, Massachusetts) using unmodified nucleosides, followed by digestion with Turbo DNAse (Life Technologies, Carlsbad, California) to remove template DNA, and capped using a Vaccinia capping...
RNA degradation and contamination were assessed using 1% agarose gel electrophoresis. A total amount of 1 μg RNA per sample was used as input material to generate sequencing libraries using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, Ipswich, MA, USA), following the manufac...
Briefly, total RNA was extracted with TRIzol reagent, followed by DNase (NEB, Ipswich, MA) treatment to remove DNA. An iScriptTM cDNA synthesis kit (Bio-Rad, Hercules, CA) was used to synthesize cDNA. Quantitative Real-Time PCR (q-RT-PCR) was performed using a CFX RT-PCR detection ...
(NEB, Ipswich, MA), 2 U of RNase H (Invitrogen) and 30 nmol of deoxyribonucleotides, followed by a 2.5 hour of incubation at 25 °C, was used to generated the second cDNA strand. For an optimal quality, QIAquick PCR purification kit (Qiagen) was employed to enrich the PCR ...
After the PCR products were cloned into the pMECS vector using the T4 ligation enzyme (NEB, Ipswich, MA, USA), the positive plasmids were electro-transformed into competent E. coli TG1 cells at 1.8 kV, 2.5 μF and 200 Ω by ten 5 mm cuvettes on ice. The transformed bacterial cells ...