Prior to kinase analysis, NLK c-Myb, Raptor, Smad2, and Smad3 were immunopurified by Catch and Release® V2.0 Reversible Immunoprecipitation System and biotinylated as per manufacturer’s instructions (EZ Link™ NHS Biotin; Thermo Scientific) and immobilized on Pierce® NeutrAvidin-coated 96-...
As diagnosing KD currently relies on clinical signs, various nonspecific clinical symptoms can also manifest, such as irritability, uveitis, aseptic meningitis, pyuria, arthritis, hypoalbuminemia, impaired liver function, abdominal pain, hydrops of the gallbladder, anemia, and shock [5]. Among such...