Abbud‑Antaki RA, Marhefka JN, DeLuca AL and Zuromskis MP: The cancer biochip system: a functional genomic assay for anchorage‑independent three‑dimensional breast cancer cell growth. Horm Cancer 3: 261‑270, 2012.Abbud-Antaki RA, Marhefka JN, Deluca AL, Zuromskis MP (...
Until now, the identity of the presumed prostate cancer stem cell has been illusive and the efforts to characterize such cells have been hampered by the lack of efficient prostate stem cell activity assay. Anchorage-independent growth of brain and mammary stem cells in vitro has been proved to...
The mammosphere assay was developed to identify the breast stem cells using either normal breast cells from reduction mammoplasties or various breast cancer cell lines to examine various stem cell regulatory pathways.39–42 These anchorage-independent growth conditions have also been demonstrated to ...
We performed anchorage-independent cell growth assay with these stable transfectants. The results indicated that overexpression of RhoGDIα-WT resulted in significant reduction in colony numbers as compared with those in control vector-transfected cells (Figure 1C). Interestingly, in contrast to RhoGDI...
Previous work has characterized the process of anchorage-independent growth of cancer cells in vitro as a key aspect of the tumor phenotype, particularly with respect to metastatic potential. Nevertheless, it remains a major challenge to translate these cell biology findings into the context of human...
In 1975, Folkman and Greenspan demonstrated the importance of anchorage for cell growth control [5]. The following researchers proved the importance of anchorage-independent survival in normal tissue dynamics and cancer progress. Four different forms of anchorage-independent survival, including apoptotic ...
or cell harvesting Assay for thymidine incorporation into anchorage dependent or independent cells without filtration or cell harvestingAssay for thymidine incorporation into anchorage dependent or independent cells without filtration or cell harvestingDNA synthesis...
Furthermore, RNA interference-mediated reduction of Pyk2 and PECAM-1 protein levels reduced cell aggregation and inhibited the growth of tumor cells in soft agar. Conclusions The data demonstrated that Pyk2 and PECAM-1 were critical mediators of both anchorage-independent growth and anoikis resistance...
This cell line showed enhanced proliferation and anchorage-independent growth as revealed by MTT assay and soft agar colony formation assay. The percentage of cells in S and G2 phases increased in transfected cell line. CONCLUSION: L02 cell line stably expressing the HBx deletion mutant is ...
Clonogenic assays under either anchorage-dependent or -independent conditions are very useful for testing the sensitivity of tumor cells to cytotoxic drugs and radiation. These assays have not been widely used with squamous-cell carcinomas (SCC) because of poor tumor-cell viability and poor cloning ...