Persistent HPV16 infection is a major cause of the global cancer burden. The viral life cycle is dependent on the differentiation program of stratified squamous epithelium, but the landscape of keratinocyte subpopulations which support distinct phases of
Quantitative expression data were normalized to the mitochondrial transcript MT-ND6 using the MT-ND6 Fw 5′-GGGTTGAGGTCTTGGTGAGT-3′ and MT-ND6 Rv 5′-ACCAATCCTACCTCCATCGC-3′ primers. TMX1, SSR2, PSK1N and MT-ND6 primers were obtained from ref. 9....
You can read more about such models and techniques in the book, “The Statistical Analysis of Failure Time Data” by Kalbfleisch and Prentice (Wiley-Interscience, 2002), at bit.ly/2TACdLR. I’ll make the assumption that each maintenance operation performed on a machine component completely ...
You can read more about such models and techniques in the book, “The Statistical Analysis of Failure Time Data” by Kalbfleisch and Prentice (Wiley-Interscience, 2002), at bit.ly/2TACdLR. I’ll make the assumption that each maintenance operation performed on a machine component completely ...
How a single cell ultimately gives rise to a patterned, complex organism is a fundamental question in biology. Embryonic development can be generalized as a process of progressive restriction of cellular potential. In vertebrates, the zygote is totipotent, but by blastula stages the three primary ge...
Height and weight of patients A and B at first referral to our hospital, 3 years and 8 months, and 10 years and 3 months, respectively Table 2. Biochemical findings in the seven patients PatientNa mmol/LK mmol/LCl mmol/LMg mmol/LpH[HCO3‒] mmol/LBEPRA ng/mL/hrAldo pg/mlCa/Cr ...
For the analysis of Trp8Arg (TGG/CGG) and Ile15Thr (ATC/ACC) variants, the 797 bp region of exon 2 was amplified using the primers forward, 5′-TCT TTG TGG GTG GTG TAC CAC GC-3′, and reverse, 5′-GGA GGA TCC GGG TGT CAG GGC TCC A-3′. The PCR product was digested by ...
Mutational Analysis of the Myxovirescin Biosynthetic Gene Cluster Reveals Novel Insights into the Functional Elaboration of Polyketide Backbones It has been proposed that two acyl carrier proteins (ACPs)—TaB and TaE—and two 3-hydroxy-3-methylglutaryl synthases (HMGSs)—TaC and TaF—could constit...
The routine 2-DE approach allows the detection of a limited number of protein spots compared with iTRAQ and other high-throughput quantitative proteomic methods30. It is considered that 2D PAGE can resolve up to only 5000 protein spots simultaneously, depending on the gel size and pH gradient ...
Prostate cancer is the second most common malignancy in men worldwide and consists of a mixture of tumor and non-tumor cell types. To characterize the prostate cancer tumor microenvironment, we perform single-cell RNA-sequencing on prostate biopsies, prostatectomy specimens, and patient-derived organo...