Persistent HPV16 infection is a major cause of the global cancer burden. The viral life cycle is dependent on the differentiation program of stratified squamous epithelium, but the landscape of keratinocyte subpopulations which support distinct phases of
Prostate cancer is the second most common malignancy in men worldwide and consists of a mixture of tumor and non-tumor cell types. To characterize the prostate cancer tumor microenvironment, we perform single-cell RNA-sequencing on prostate biopsies, prostatectomy specimens, and patient-derived organo...
(Nelumbo) and functional study of NnbZIP36 in regulating anthocyanin synthesis Ping Zhou1, Jingwen Li2, Huiyan Jiang1, Qijiang Jin1, Yanjie Wang1 and Yingchun Xu1* Abstract Background The basic leucine zipper (bZIP) family is a predominant group of ...
For the analysis of Trp8Arg (TGG/CGG) and Ile15Thr (ATC/ACC) variants, the 797 bp region of exon 2 was amplified using the primers forward, 5′-TCT TTG TGG GTG GTG TAC CAC GC-3′, and reverse, 5′-GGA GGA TCC GGG TGT CAG GGC TCC A-3′. The PCR product was digested by ...
You can read more about such models and techniques in the book, “The Statistical Analysis of Failure Time Data” by Kalbfleisch and Prentice (Wiley-Interscience, 2002), at bit.ly/2TACdLR. I’ll make the assumption that each maintenance operation performed on a machine component completely ...
pH 7.20, PCO250 mm Hg, PO280 mm Hg, and an O2saturation of 95%. This patient has a mixed metabolic and respiratory acidosis. This is evident, because one would expect a patient who has this degree of respiratory acidosis to have a pH of 7.32 if this was a pure respiratory acidosis....
How a single cell ultimately gives rise to a patterned, complex organism is a fundamental question in biology. Embryonic development can be generalized as a process of progressive restriction of cellular potential. In vertebrates, the zygote is totipotent, but by blastula stages the three primary ge...
Cells have the ability to respond and adapt to environmental changes through activation of stress-activated protein kinases (SAPKs). Although p38 SAPK signalling is known to participate in the regulation of gene expression little is known on the molecular mechanisms used by this SAPK to regulate st...
Quantitative expression data were normalized to the mitochondrial transcript MT-ND6 using the MT-ND6 Fw 5′-GGGTTGAGGTCTTGGTGAGT-3′ and MT-ND6 Rv 5′-ACCAATCCTACCTCCATCGC-3′ primers. TMX1, SSR2, PSK1N and MT-ND6 primers were obtained from ref. 9....
Spectrophotometry was used to test TAC (U/L) and MDA (nmol/mL) levels. MDA levels were determined using the thiobarbituric acid (TBA) method. Semen samples were centrifuged at 4 °C for 15 min with a speed of 2000 r/min. The supernatant was mixed with the reagents supplied in an MDA...