Persistent HPV16 infection is a major cause of the global cancer burden. The viral life cycle is dependent on the differentiation program of stratified squamous epithelium, but the landscape of keratinocyte subpopulations which support distinct phases of
Quantitative expression data were normalized to the mitochondrial transcript MT-ND6 using the MT-ND6 Fw 5′-GGGTTGAGGTCTTGGTGAGT-3′ and MT-ND6 Rv 5′-ACCAATCCTACCTCCATCGC-3′ primers. TMX1, SSR2, PSK1N and MT-ND6 primers were obtained from ref. 9....
pH 7.20, PCO250 mm Hg, PO280 mm Hg, and an O2saturation of 95%. This patient has a mixed metabolic and respiratory acidosis. This is evident, because one would expect a patient who has this degree of respiratory acidosis to have a pH of 7.32 if this was a pure respiratory acidosis....
Explants ofXenopuspluripotent blastula cells have also played a central role in determining the signals that control formation of the other embryonic germ layers, mesoderm and endoderm [49,50,51,52]. Nodal, Activin, and Vg-1, ligands of the other branch of the TGF-beta family, have been sho...
Thermal Analysis is defined as a technique where a substance's property is determined based on the programmed temperature changes applied externally, allowing for the monitoring of endothermic and exothermic processes such as melting, boiling, crystallization, and chemical degradation. ...
0,2 μL Platinum Taq DNA polymerase, 100 ng of extracted DNA and 25 pmoles of the primers PPP-1 5'-gtc gtc cac gat gag gag ct-3' and PPP-4 5'-tac gtg gga agc gcc tcg ct-3' (based on the previously published sequence of the B2L gene of Orf virus strain NZ2/2004 [12])...
(12,000g; 15 min). Precipitation was performed by adding 1/10 volume of sodium acetate (3 M; pH 5.2) and 3 volumes of ethanol (~99%) to the supernatant and incubation at −20 °C overnight. Afterwards, each sample was centrifuged (12,000g; 20 min). The pellet was washed two ...
Cells have the ability to respond and adapt to environmental changes through activation of stress-activated protein kinases (SAPKs). Although p38 SAPK signalling is known to participate in the regulation of gene expression little is known on the molecular mechanisms used by this SAPK to regulate st...
Prostate cancer is the second most common malignancy in men worldwide and consists of a mixture of tumor and non-tumor cell types. To characterize the prostate cancer tumor microenvironment, we perform single-cell RNA-sequencing on prostate biopsies, prostatectomy specimens, and patient-derived organo...
Stem cells are attractive candidates for the regeneration of tissue and organ. Mesenchymal stem cells (MSCs) have been extensively investigated for their potential applications in regenerative medicine and cell therapy. For developing effective stem cell