Genome-wide analysis of gene expression is the study of transcription at a genomic scale, also known as transcriptomics. Typically relying on data from microarrays or high throughput sequencing, it can determine what genes or transcript isoforms are enriched in a particular cell or tissue type, con...
analysis of gene expression. Invest Ophthalmol Vis Sci 43: 1048-1058, 2002.Burgess J K,McParland B E.Analysis of gene expression. Methods in Enzymology . 2002Bosetti, F., Seemann, R., Bell, J.M., Zahorchak, R., Friedman, E., Rapoport, S.I., Manickam, P., 2002. Analysis of...
基因表达系列分析（SerialAnalysisofGeneExpression,SAGE）技术 SAGE 技术 MRNA 结合到微珠子上(Microscopic Bead and mRNA)mRNA 转录成DNA(mRNA binds to bait and is copied into DNA)
geneexpressionmonitoring,DNAmicroarrayshavecomealong way.FromafewdozencDNAsspottedonglassornylon membranestothecompletionofthe‘fullgenomechip’forS. cerevisiae,wehavereachedapointatwhichgenome-wide monitoringofexpressionlevelsofallhumanandmousegenes seemscloseathand.Withaworkingdraftofthehumangenome ...
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基因表达系列分析 1995 年 Velculescu 等提出了基因表达系列分析 (Serial Analysis of Gene Expression,SAGE)技术,能同时对上千个转录物进行研究。 1. SAGE 的原理和实验路线。 1.1 SAGE 的原理 SAGE 的主要依据有两个。第一,一个 9～10 碱基的短核苷酸序列标签包含有足够的信息, 能够唯一确认一种转录物。例如...
Gene expression changes frequently mediate the actions of many drugs and developments in the gene expression profiling and proteomics are being increasingly used to delineate drug mechanisms. Gene expression data differ considerably from typical pharmacokinetics and pharmacodynamics data sets in their size, ...
Serial analysis of gene expression(SAGE) is a method for generatingexpressionprofiles of the mRNAs in a given cell type[21]and can be adapted for imprinted gene detection. SAGE is not dependent on the prior availability of transcript information, and it can detect relatively low level...
In this study chickpea (Cicer arietinum), a popular food legume crop was used for analysis of gene expression under drought stress. Although chickpea is generally grown in relatively less irrigated lands and some cultivars adapt well to the water-limited environment [21], drought poses a serious...
Reverse Transcription, coupled with the Polymerase Chain Reaction (RT-PCR), has literally revolutionized the study of gene expression. It is now possible to detect the RNA transcript of any gene, regardless of the amount of starting material or the relative abundan...