Ion mobility mass spectrometryNative mass spectrometrySize exclusion chromatography-native mass spectrometryMass spectrometry performed in nondenaturing conditions (native MS) has proven its utility for the quantitative and qualitative analysis of antibody-drug conjugates (ADCs), especially when ADCs'......
purification, and characterization. With the continuous development ofantibody-drug conjugates (ADCs), HIC is becoming increasingly widely used in thecharacterization and analysis of ADCs. Unlike other techniques, HIC allows proteins to be analyzed under mild non-denaturing conditions while maintaining ...
The complexity of modern therapeutic proteins, such as antibody-drug conjugates (ADCs), presents a significant analytical challenge requiring high resolution chromatography combined with high resolution mass spectrometry. ADCs involving cysteine conjugation pr...
Protein analysis in native or native-like conditions decreases charge state value resulting in mAb detection at higher m/z ranges with more spatial resolution. For example, native mass spectrometry conditions also allow the preservation of structur...
High-Throughput Size Exclusion Chromatography Analysis of Monoclonal Antibodies - 5994-0828EN 29 Sensitive Native Mass Spectrometry of Macromolecules Using Standard Flow LC/MS - 5994-1739EN 33 Analysis of Camelid Single-Domain Antibodies Using Agilent AdvanceBio SEC 120 Å 1.9 μm and AdvanceBio ...
Mass Spectrometry. Waters is the leading provider of lab equipment, supplies and software for scientists across the world. Easily research and order everything your lab needs! Waters offers a comprehensive range of analytical system solutions, software, and services for scientists. Liquid Chromatography...
State-of-the-Art native mass spectrometry and ion mobility methods to monitor homogeneous site-specific antibody-drug conjugates Synthesis Pharmaceuticals, 14 (2021) Google Scholar [23] F. Fussl, L. Strasser, S. Carillo J. Bones, Native LC-MS for capturing quality attributes of biopharmaceuticals...
LC-MS measurement on intact ADCs demonstrated great potential in the literature for DAR analysis of all kinds of ADCs with ESI-(Q)TOF-MS8, 29, 31, native MS32, and ion mobility MS32, CE-MS33, etc. The approach using ESI-(Q)TOF-MS for intact ADCs detection8, 29, 31 after Fc ...
The goal of this review is to provide a general overview of theoretical and practical aspects of modern HIC applied for the characterization of therapeutic protein biopharmaceuticals including monoclonal antibodies (mAbs), antibody drug conjugates (ADCs) and bispecific antibodies (bsAbs). Therefore, ...
Analysis and characterization of protein aggregates: The services provided by our experts include a range of protein aggregation analysis techniques that are used to detect and quantify aggregates in solution in support of formulation...