This unit gives a brief description of the methods used for the cultivation of anaerobic microorganisms, and describes in detail the principles and applications of anaerobic chambers, with special emphasis on vinyl glove boxes. The methodologies described in this unit should provide the interested but...
BryantMP (1972) Commentary on the Hungate technique for culture of anaerobic bacteria. Am J Clin Nutr 25: 1324–1328 Google Scholar ClesceriLS (ed) (1989) Standard methods for the examination of water and wastewater. American Public Health Association, Washington, D.C. Google Scholar CookGM...
aeruginosa isolates from chronically infected CF adults were compared with strain PAO1 and other clinical non-CF isolates for biofilm formation and phenotypic properties related to biofilm growth under aerobic and anaerobic culture conditions. Materials and methods Top of page Abstract Introduction ...
Optimal techniques for isolation ofanaerobic bacteriaare described inChapter 3. The decision to request anaerobicbacterial cultureshould be considered carefully, because anaerobes are difficult to culture, tend to have predictable susceptibility to antimicrobial drugs, and can contaminate specimens, which can...
For the planktonic cell culture, the cells were obtained after 14 h of cultivation in M63 medium. The media were then replaced with fresh medium containing antibiotics and dispensed in a 96-well polystyrene microplate. After 1 h of incubation, viable cells were counted. For the biofilm ...
Infection and Drug Resistance Dovepress open access to scientific and medical research Open Access Full Text Article ORIGINAL RESEARCH Effects of Blood Culture Aerobic/Anaerobic Bottle Collection Patterns from Both Sides of the Body on Positive Blood Culture Rate and Time-to-Positivity Hainan Wen1,*,...
Maximum amount of sulfide recovered in HSR6 culture was 14 mM with a concomitant consumption of 15 mM formate from 30 mM supplied, well corresponding to the 2 electron reduction of zero-valent sulfur. No further growth of HSR6 was observed likely due to the inhibition by accumulating sulfide....
Methods Reactor setup and operation Three continuous membrane bioreactors (working volume, 2 l) were inoculated with an equal amount (1 l, OD600 = 0.8) of highly enriched (more than 95% K. stuttgartiensis cells18) free-living planktonic cell suspensions of the anammox bacterium K. ...
M. nitroreducens’-dominated culture with either nitrate or iron as the sole terminal electron acceptor, and a series of characterisations on MHCs were conducted. The AOM activities observed (based on 13CO2 production rates from fed 13CH4) when incubated with soluble iron (ferric citrate) or ...
Non-isotopic methods to assess anaerobic biodegradation of ETBE and TBA Isotopic methods to assess anaerobic biodegradation of ETBE and TBA Conclusion and research directions References Acknowledgements Funding Author information Ethics declarations Additional information Rights and permissions About this article ...