India) [42]. For aerobic bacterial counts, R2A and MSM agar plates were incubated at 30 °C for 2 days. For anaerobic bacterial counts, anaerobic agar plates were incubated at 30 °C for 15 days in an anaerobic jar with the anaerobic gas pack (...
One was for anaerobic culture using Brucella blood agar incubated in an anaerobic jar that secured anaerobiosis using the anaerobic gas pack. The other swab was used for aerobic culture on blood agar incubated on air at 37oC. Identifications and antibiotic sensitivity testing were performed according...
this does not happen and glycerol fermentation proceeds optimally. By increasing headspace, H2 was diluted and glycerol fermentation was improved. Flushing the headspace with inert gas or CO2 removes excess H2 and further increases glycerol fermentation. Sparging, or bubbling gas through the culture, ...