The PolyTiter immunofluorescent titration system (PolyTiter system), an attachment for conventional fluorescent microscopes, facilitates immunofluorescent antinuclear antibody (IFA-ANA) testing by using calibrated light attenuation to determine the semiquantitative endpoint dilution or titer of ANA-positive ...
The “gold standard” serologic test for diagnosis of HGA in humans is an indirect immunofluorescence assay (IFA). This assay must be performed at multiple time points over a period of several weeks and can only be performed in specialized reference laboratories. A limitation of the IFA assay ...
The primary immunization is prepared 1:1 in Freund's Complete Adjuvant (FCA); subsequent boosts are prepared in Freund's Incomplete Adjuvant (IFA). At day 20, test bleeds are taken and screened by ELISA, to determine the antibody titer. If the titers from any of the mice are sufficiently ...
computer-aided immunofluorescence microscopyThe assessment of antinuclear antibodies (ANA) by indirect immunofluorescence (IFA) allows detection of >50 characteristic autoantibodies, making it a highly sensitive method and screening test. The semiquantitative method is based on the identification of patterns ...
IFA is designed to assess increases in both IgM and IgG. While IgG antibody responses can be very specific, IgM responses are less so and have the potential to yield false-positive test results. Enzyme immunoassay (EIA) are also used for diagnosis. EIA tests are not quantitative and provide...