ANA can be detected by indirect immunofluorescence assays (IFA) on HEp-2 cells fixed to glass slides or by immunoassays, e.g. ELISA or immunoblot. In ANA detection IFA and immunoassays complement each other: one test is used for screening, the other for confirmation of positive or ambiguous ...
First, we have analyzed agreement (kappa index) between qualitative (positive or negative) results obtained with both assays using various cut-off. Then, IFA-ANA titers were considered. Box plots show that when IFA titer increases, COBAS-ANA value does Discussion We evaluated -precision, response...
A total of 172 consecutive ANA-IFA positive serum specimens, including the four most common pattern types, were evaluated in parallel by two readers to determine the dilution endpoint. The two methods produced equal dilution endpoints in 78 specimens (45%), within 1-dilution endpoint in 121 ...
In the case of Sjgren's syndrome and scleroderma patients the COBAS Core EIA yielded a lower rate of positive results compared to IFA. This discrepancy may be explained by the lack of detection of autoantibodies to nuclear antigens that can be identified only by IFA due to their ...
, which uses a recombinant Msp2/p44 protein, is also available for the detection of antibodies toAnaplasmaspecies in dog serum. Positive results obtained with these ELISA assays do not imply thatA. phagocytophilumis the cause of illness, and as with IFA testing, negative results...
1 x 0.5 mL Positive Control Serum 1 x 0.5 mL Negative Control Serum 1 x 3.5 mL Conjugate 1 x 3.5 mL Mounting Medium 2 x1 liter packets PBS Code Number Product Name Size ANK-120 ANA (HEp-2) Test System 120 Tests ANK-60 ANA (HEp-2) Test System ...
Conclusions ANA tests requested in rheumatology clinic can be false positive up to %35. However, only 12.3% of them had a titer ≥1/320. The sensitivity of the ANA in patients with SLE as assessed by IFA was more than 98%. Nuclear membrane and DFS70 patterns are not specific to any ...
(defined as confirmed cases of anaplasmosis), or by the presence of positive IFA titer > or = 1: 256 in acute and convalescent phase serum samples ... Ljiljana Misi-Majerus,Nevenka Buji,Vesna Madari,... - 《Acta Med Croatica》 被引量: 34发表: 2006年 Advancing Integrated Tick Managemen...
Because Anaplasmosis andBorrelia burgdorferi(Lyme)are spread by the same ticks, patients who test positive forBorrelia burgdorferishould also be tested for antibodies toAnaplasma phagocytophilum. Want to read more about Anaplasmosis?Visit the CDC’s information page....
The Anaplasma IgG IFA titre showed a twofold increase (testing for IgM was not performed). Moreover, there was a decline of the ELISA Borrelia IgM levels from > 200 U/ml to 121 U/ml (positive cut-off > = 22 U/ml) confirmed by immunoblot in both cases, whereas the ...