During the last decade, the International Consensus on ANA Patterns (ICAP) initiative promoted harmonization and understanding of HEp-2 IFA staining pattern nomenclature, as well as promoting their use in patient care by providing interpretation for HEp-2 IFA test results. In conjunction with a ...
a) Homogeneous pattern on IFA b) Most commonly seen in drug-induced lupus c) In drug-induced lupus there is not normally n association with other autoantibodies 15 Anti-Nuclear Antibody (ANA) 4) Anti-RNP antibodies (Speckled or Nucleolar Pattern) ...
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The antinuclear antibody HEp-2 indirect immunofluorescence assay: a survey of laboratory performance, pattern recognition and interpretation Background To evaluate the interpretation and reporting of antinuclear antibodies (ANA) by indirect immunofluorescence assay (IFA) using HEp-2 substrates b... AE Tebo...
Even if a low cut-off value (1:64) was used, this high seroprevalence is sustained by another study from Belgium [96]. Among 1350 patients suspected of a tick-borne infection between 2000 and 2009, 418 (31%) of patients were found positive for either IgG or IgM antibodies, using IFA ...
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A total of 172 consecutive ANA-IFA positive serum specimens, including the four most common pattern types, were evaluated in parallel by two readers to determine the dilution endpoint. The two methods produced equal dilution endpoints in 78 specimens (45%), within 1-dilution endpoint in 121 ...
Use of Computer-Aided Immunofluorescence Microscopy (CAIFM) for Interpretation of Antinuclear Antibody (ANA) Pattern and Titerantinuclear antibodyautoimmune diseasedigital pathologycomputer-aided immunofluorescence microscopyThe assessment of antinuclear antibodies (ANA) by indirect immunofluorescence (IFA) allows ...
Some of these are outlined in this article, including the planning of three audio-visual educational modules to be posted at the www.anapattern.org website, the classification of two novel HEp-2 IFA patterns, the implementation of a project dedicated to continuously updating the information on ...
However, the presence of less common antibodies such as anti-fibrillarin (U3-RNP) that generate a clumpy nucleolar pattern by HEp-2 indirect immunofluorescence assay (IFA, ICAP AC-9) are considered disease specific and are with clinical subsets of SSc, therefore playing a role in diagnosis and...