Immunofluorescence on HEp2-cells is the standard diagnostic assay for the detection of anti-nuclear antibodies (ANA). Cytoplasmic speckled patterns are a common finding, and are associated with various antibodies, including anti-synthetase antibodies. However, classic ENA testing generally identifies only...
Most nucleolar patterns are associated with nuclear patterns.CAUTION: falsely positive nucleolar patterns are among the most common artifacts in HEp-2 testing and are usually caused by: ?excess of counterstain; this problem is easily solved by decreasing counterstain concentration or by working without...
Sixty two sera displaying a DFS70-like fluorescence pattern at a titer equal to or higher than 1:160 in the ANA test on HEp-2 cells were studied. Sera came from a cohort of samples referred as possible DFS70-positive ANA by expert clinical pathologists, collected consecutively from routine ...
%PositiveDisease 5 Anti-NuclearAntibody(ANA) HomogeneousPattern 6 Anti-NuclearAntibody(ANA) RimPattern CLS420ClinicalImmunology&MolecularDiagnostics–LectureSeries Immunity&AutoimmuneDiseases2 7 Anti-NuclearAntibody(ANA) SpeckledPattern 8 Anti-NuclearAntibody(ANA) ...
The DFS pattern on HEp-2 cells is well differentiated from the classic "homogeneous" ANA pattern associated with dsDNA antibodies. In DFS pattern positive sera the most important detectable ANA specificity is the DFS70 antibody (synonym LEDGF antibody). This antibody is...
Ninety-two cases (3.4% of the total cases) were analyzed using Microblot-Array ANA plus, with reticular as the most frequent cytoplasmic pattern, followed by dense fine speckled. The most frequently associated disease with reticular pattern was primary biliary cholangitis (28.9%), and the most ...
ANA titer (positive 鈮 1:160) and cellular staining patterns were assessed by indirect immunofluorescence (Hep-2 cells). Results Ages ranged from 85 to 98 years, with 58.8% being females. The prevalence of ANA in this population was 61.5%, mostly in lower titers (1:160 in 45.0%, 1:...
The positive ANA patterns included AC2 – Speckled, AC3 – Centromere, AC6 – Discrete Nuclear Dots, AC7 – Discrete Nuclear Dots, and AC8 – Nucleolar patterns separately. No AC-29 - DNA topoisomerase I was examined and resulted. We found that Hong Kong have a higher distribution of ANA...
SAT0560Automated Ifa Methods Compare Well with Established Manual IFA Screening and Titration for ANA HEp-2BackgroundOften, only basic nuclear patterns, like homogenous, speckled, nucleolar, centromere or cytoplasmic, are reported by laboratories. The detection of other patterns requires well trained ...
Indeed, this pattern is one of the most frequently observed in non-autoimmune patients with an ANA-HEp-2 positive reaction. The present review intends to provide elements allowing the clinician to perform a critical interpretation of results of an ANA-HE-2 test, including analysis of the titer...