The next day, the samples were washed twice in TF-wash buffer I (20 mM Tris–HCl pH 7.4, 150 mM NaCl, 0.1% SDS, 1% Triton X-100 and 2 mM EDTA pH 8.0), followed by two washes in TF-wash buffer III (10 mM Tris–HCl pH 8.0, 250 mM LiCl, 1% Triton X-100...
For α-amylase activity test, the protein samples were separated by SDS-PAGE containing 1% soluble starch. After electrophoresis, SDS was removed by washing the gel with water followed by 10 min incubation at room temperature. This was repeated twice. The gels were then immersed in the ...
In SDS gel electrophoresis of proteins or electrophoresis of polynucleotides, mobility depends primarily on size and is used to determined molecular weight. In pulse-field electrophoresis, two fields are applied alternately at right-angles to each other to minimize diffusion-mediated spread of large ...
The criteria for successful photocoagulation are the presence of visible bubbles, indicating that Bruch’s membrane (a layer of the retina) has been disrupted. The purity of rAAV capsids was measured by SDS-PAGE 60 μL of rAAV samples at a concentration of 1.0 × 1012 vg/mL were mixed...
Additional evidence of the multiplicity of the halo has been provided by An et al. (2013). Using a metallicity calibration from SDSS photometry, they found that candidate halo stars with retrograde motion are more metal-poor than those with pro-grade motion, which indicates that the outer ...
This article has beenupdated Abstract This study used theinstructional humor processing theoryto test how different humor subtypes employed by teachers (course-related, course-unrelated, self-disparaging, other-disparaging) relate to students’ well-being, sense of belonging, and engagement. The particip...
Ever since the recent completion of the peach genome, the focus of genetic research in this area has turned to the identification of genes related to important traits, such as fruit aroma volatiles. Of the over 100 volatile compounds described in peach,
The nuclear lysate was resuspended in SDS lysis buffer (1%SDS, 5 mM EDTA, 50 mM Tris/HCl (pH8.1), 1× CompleteMini protease inhibitor (Roche)). Chromatin was sheared to an average fragment length of 200–1200 bp by sonication on ice (Branson sonifier 250). Chromatin immunoprecipitation (...
Human SDs are organized into an estimated 435 duplication blocks ranging in size from 50 kbp to multiple Mbp in length. Their ancestral reconstruction has revealed a highly nonrandom organization with respect to both chromosomal distribution and their structure. The 435 duplication blocks can be group...
Cell lysates were prepared by lysing cells in ice-cold RIPA buffer (25 mM Tris–HCl pH 7.6, 0.15 M NaCl, 1% IGEPAL CA-630, 1% sodium deoxycholate and 0.1% SDS) containing 1× protease inhibitors (Thermo Scientific). Lysate was cleared by centrifugation at 20,000g for 10 min at...