Ribosomal RNAs (rRNAs) 100–150, 1500–5000 (depending on rRNA molecule) All organisms Catalyze protein translation Signal recognition particle RNA (7SL RNA or SRP RNA) 300 All organisms Targeting of proteins to membranes during translation Small nuclear RNAs (snRNAs) 100–150 Eukaryotes Splicing ...
Initiation of ColE1 replication starts with the transcription of an RNA molecule of 555 bases that can act as a primer for DNA synthesis. This pre-primer RNA (sometimes called RNAII) is cleaved by ribonuclease H to generate a primer with a free 3′-OH group, which can be used by DNA ...
Comparative analyses revealed a small RNA secondary structural element, designated the GA motif, that is highly conserved in both T box and S box leader sequences. The motif consists of two short helices separated by an asymmetric internal loop, with highly conserved GA dinucleotide sequences on ...
A Variant Surface Glycoprotein (VSG) coat protects bloodstream form Trypanosoma brucei. Prodigious amounts of VSG mRNA (~7-10% total) are generated from a single RNA polymerase I (Pol I) transcribed VSG expression site (ES), necessitating extremely high
RNA is a thermodynamically stable molecule, which is, however, rapidly digested in the presence of the nearly ubiquitous RNase enzymes. As a result, shorter fragments of RNA commonly occur in a sample, which can potentially compromise results of downstream applications [1, 2]. In order to evalu...
The discovery of ribozymes has inspired exploration of RNA’s potential to serve as primordial catalysts in a hypothesized RNA world. Modern oxidoreductase enzymes employ differential binding between reduced and oxidized forms of redox cofactors to alter
The term RNA editing describes those molecular processes in which the information content is altered in an RNA molecule. To date such changes have been observed in tRNA. rRNA and mRNA molecules of eukaryotes, but not prokaryotes. The demonstration of RNA editing in prokaryotes may only be a ...
PCR amplification of reverse transcribed Brucella RNA using the pairs of primers indicated in Table 1 was conducted to assess the continuity of the transcript until we reached the first gene annotated on the opposite strand (BAB1_1389). Genomic DNA and total RNA were used as positive and ...
Here, we report the first isolation and characterization of a cellulose synthase gene from the commercially valuable Acacia hybrid with the aim of understanding the deposition of cellulose in its primary and secondary cell walls. Materials and Methods Plant Material and Total RNA Preparation Young ...
The genome of the virus is an RNA molecule of about 7.4 kb that codes for a single polyprotein that is cleaved to produce the various proteins that are required for virion structure and replication. The RNA is polyadenylated at the 3′ end while the 5′ end is covalently bound to a sm...