Small Hairpin RNA (shRNA) refers to a type of RNA molecule that can target and silence specific viral proteins. It has been extensively studied by researchers for its antiviral effects and its ability to silence different strains of alphaviruses. It works by degrading the viral single-stranded ...
Ribosomal RNAs (rRNAs) 100–150, 1500–5000 (depending on rRNA molecule) All organisms Catalyze protein translation Signal recognition particle RNA (7SL RNA or SRP RNA) 300 All organisms Targeting of proteins to membranes during translation Small nuclear RNAs (snRNAs) 100–150 Eukaryotes Splicing ...
The discovery of natural RNA enzymes (ribozymes)1,2and their presence in all three domains of life2,3has inspired researchers to explore the potential of RNA molecules to serve as primordial catalysts. A hypothesized RNA world4could have made extensive use of diverse RNAs to support replication ...
A Variant Surface Glycoprotein (VSG) coat protects bloodstream form Trypanosoma brucei. Prodigious amounts of VSG mRNA (~7-10% total) are generated from a single RNA polymerase I (Pol I) transcribed VSG expression site (ES), necessitating extremely high
RNA is a thermodynamically stable molecule, which is, however, rapidly digested in the presence of the nearly ubiquitous RNase enzymes. As a result, shorter fragments of RNA commonly occur in a sample, which can potentially compromise results of downstream applications [1, 2]. In order to evalu...
If a codon on an mRNA molecule is GGA, what is the anticodon on its matching tRNA molecule? a) GGU b) CCT c) AAC d) CCU tRNA Molecule A particular class of RNA molecule known as tRNA (transfer RNA) is essential for the production of ...
PCR amplification of reverse transcribed Brucella RNA using the pairs of primers indicated in Table 1 was conducted to assess the continuity of the transcript until we reached the first gene annotated on the opposite strand (BAB1_1389). Genomic DNA and total RNA were used as positive and ...
The mature and active insulin protein has a molecular mass of approximately 5.800 kDa and includes two polypeptide chains (A- and B-chain) that are linked by a disulfide bond [16]. A specific antibody that reacts only with the intact insulin molecule (not A-chain, B-chain or pro-insulin...
protein translation, carbohydrate (glucose) metabolism, RNA processing/splicing, and RNA/protein transport, followed by macromolecular complex and organelle organization, regulation of cell cycle and cell death, chromosome organization and transcription, regulation of protein and other small molecule ...
[2,32]. Hormone receptors normally function as homo- or hetero-dimers and each monomer typically consists of ligand-binding, DNA-binding, and transcription regulatory domains. The zinc-coordinating motif is found in the DNA-binding domain and is characterized by two antiparallel a helices capped ...