Unsaturated ionizable lipids have also been demonstrated to enhance mRNA delivery. Anderson and colleagues showed a linoleic acid-derived ionizable lipid (OF-02) achieved the highest hepatic mRNA delivery and protein expression among its class7. Later, they demonstrated that an alkyne ionizable lipid...
Herein, we design a library of ionizable cationic LNPs with different amine headgroups for brain-targeted siRNA delivery (Scheme 1). Using in vitro and in vivo screening approaches, a leading lipid, 9-O16B (called BAMPA-O16B) with pKa value around 6.5, was identified as an efficient carri...
In order to introduce an antigen, a carrier for mRNA transfection is required, since the mRNA molecule per se is unstable in serum-containing medium. We previously reported on an ionizable lipid-like material with vitamin E-scaffolds (ssPalmE) as a material for a lipid nanoparticle (LNP)-...
To avoid potential toxicity from cationic lipids and to increase delivery efficiency, researchers have begun to utilize ionizable lipids. These lipids are neutral at physiological pH but become positively charged at low pH, which allows for nucleic acid–lipid complexes to form in acidic buffer. Aft...
METHOD OF SYNTHESIS OF AN IONIZABLE CATIONIC LIPIDWhat is described is a method of synthesis of the compound of formula 1AJoseph E. PaynePadmanabh Chivukula
the ionizable lipid from the ketone thereof using one or more synthesis steps resulting in an addition of an ionizable head group moiety to (a) the ketone; or (b) an alcohol produced from an optional reduction of the ketone to produce the alcohol, thereby producing the ionizable lipid. Th...
METHOD OF SYNTHESIS OF AN IONIZABLE CATIONIC LIPIDWhat is described is a method of synthesis of the compound of formula 1A, or a salt thereof, wherein R 3 is a linear or branched alkene of 1, 2, 3, 4, 5 or 6 carbons; R 4 and R 5 are the same or different, each a hydrogen,...
METHOD OF SYNTHESIS OF AN IONIZABLE CATIONIC LIPIDWhat is described is a method of synthesis of the compound of formula 1A, or a salt thereof, wherein R 3 is a linear or branched alkene of 1, 2, 3, 4, 5 or 6 carbons; R 4 and R 5 are the same or different, each a hydrogen,...
The present disclosure describes compositions, preparations, nanoparticles (such as lipid nanoparticles), and/or nanomaterials and methods of their use.HAMILTON, GREGORY, LAWRENCESHEHATA, MINA, FAWZYSAGO, CORY, DANEPATWARDHAN, NEERAJ, NARENDRACHHABRA, MILLONI, BALWANTKUMAR...
ionizable mobile carriertransport kineticslipid membranealkali cationsvalinomycinThe kinetics of K + and Na + transport across the membrane of large unilamellar vesicles (L.U.V.) were compared at two pH's, with two carriers: (222) C 10 -cryptand (diaza-1, 10-decyl-5-hexaoxa-4,7,13,16...